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  • Open Access

    ARTICLE

    Integrated GC-MS and LC-MS-Based Untargeted Metabolomics Reveals Diverse Metabolites in Fermented Pine Needles

    Yinyu Xu1, Zheng Li1, Qian Wen2, Hao He1, Pengtu Shi1, Wenhua Zhou2,*

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.047949

    Abstract The diversity of metabolites produced in fermented pine needles at different stages of fermentation has rarely been investigated. In the present study, untargeted metabolomic analysis using GC-MS and LC-MS was performed to detect metabolites in fermented pine needles at different fermentation stages. A total of 30 samples of pine needles fermented first with yeast (Saccharomyces cerevisiae) and then with a mixed bacterial culture of Lactobacillus fermentum CECT5716 and Bifidobacterium Breve M16V, were used to detect differential metabolites at different stages of fermentation. Pearson’s correlation analysis was used to determine correlations between metabolites and key microbial communities. A… More >

  • Open Access

    ARTICLE

    Effect of Ecotype and Gender on the Variation of Leaf Morphological, Epidermal and Stomatal Traits among Pistacia atlantica Desf.

    Abdelghafour Doghbage1,*, Safia Belhadj2, Hassen Boukerker3, Jean Philippe Mevy4, Thierry Gauquelin4, Alain Tonetto5, Benbader Habib1,6, Arezki Derridj7, Zahra Robã Bouabdelli1, Walid Soufan8, Fathi Abdellatif Belhouadjeb1

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.055528

    Abstract The Atlas pistachio tree is a typically Mediterranean species, which represents an important forest heritage in the arid and semi-arid regions of Algeria. It is deeply rooted in the local population’s culture, making it essential to better understand this species for its conservation and valorization. Through our work on 7 provenances of Pistacia atlantica distributed across different bioclimates in Algeria and based on 28 quantitative and qualitative leaf, trichome, and stomatal traits, it was revealed that the Atlas pistachio tree exhibits significant ecotypic variability linked to its habitat and a high adaptability to extreme conditions in… More >

  • Open Access

    ARTICLE

    Integrative Analysis of the Transcriptome and Metabolome of Poplar, Populus deltoides ‘Shalinyang’. (Salicaceae), Reveals Responses to Attack by Anoplophora glabripennis (Coleoptera)

    Jiayu Luo1,#, Jianfeng Liu1,#, Zuoxiang Sun1, Pengpeng Shao1, Danyang Wang2, Jianzhong Yao3, Binzhou Li2, Lijun Dong1, Dandan Cao1,4,*, Jianrong Wei1,*

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.052858

    Abstract Anoplophora glabripennis is one of the most devastating wood-boring beetles that attacks poplars. However, one poplar species, Populus deltoides, has strong resistance to Anoplophora glabripennis infestation, the underlying defense mechanisms against Anoplophora glabripennis are poorly understood. Secondary metabolites play a crucial role in plants to combat biological stress. Here, based on transcriptome and metabolome, we demonstrated that the mechanisms for responses to mechanical damage and insect infestation were different. The degree of reactions to adult groove production, larval incubation, and larval frass production was not identical. In addition, the potential genes with insect resistance activity were identified. Predominant differentially More >

  • Open Access

    ARTICLE

    Phytochemical and Pharmacological Research in Galenic Remedies of Solidago canadensis L. Herb

    Yurii Hrytsyk1, Oleh Koshovyi2,3, Martin Lepiku4, Valdas Jakštas5, Vaidotas Žvikas5, Tetiana Matus1, Mariia Melnyk1, Lyubov Grytsyk1, Ain Raal2,*

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.055117

    Abstract Canadian goldenrod (Solidago canadensis L.) is a rhizomatous plant of the Asteraceae family. In folk medicine, Solidago galenic remedies are used for diseases of the kidneys, urinary tract, liver, etc. Externally, goldenrod is used to treat purulent wounds, furunculosis, and gum abscesses as washes and compresses. The aims of this research were to study the yield and chemical composition of essential oil (EO), the anti-inflammatory activity of S. canadensis dry extracts based on its decoction and tincture. In EO (2.8 mL/kg) of S. canadensis were identified and quantified 34 compounds. The principal compounds of the EO from flowering tops… More > Graphic Abstract

    Phytochemical and Pharmacological Research in Galenic Remedies of <i>Solidago canadensis</i> L. Herb

  • Open Access

    ARTICLE

    Inhibitory Effect of Ponkan (Citrus reticulata ‘Ponkan’) Flavonoids on Oral Ulcers through Jak2-Stat3 Signaling Pathway

    Lili Liu1, Can Hu4, Huilin Cheng1, Chuangju Ma1, Jiancheng Sun1, Yunyi Chen2, Manxi Wu2, Han Yang2, Cui Sun2,3, Jinping Cao2,3, Dengliang Wang1,*, Yue Wang2,*, Chongde Sun2,3

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.054449

    Abstract Oral ulcers, a common mucosal affliction, severely diminish patients’ quality of life due to pain and discomfort. Despite existing treatments, the frequent recurrence of oral ulcers underscores the need for more effective therapeutic options. This study aimed to investigate the inhibitory effects of Ponkan (Citrus reticulata ‘Ponkan’) extracts and their flavonoid components on oral ulcers, exploring their potential as a natural therapy. Twenty different Ponkan cultivars were selected for flavonoid extraction and analysis. Ultra-performance liquid chromatography (UPLC) and high-resolution mass spectrometry (HRMS) were utilized for substance identification, leading to the discovery of 16 flavonoid compounds. Using… More > Graphic Abstract

    Inhibitory Effect of Ponkan (<i>Citrus reticulata</i> ‘Ponkan’) Flavonoids on Oral Ulcers through Jak2-Stat3 Signaling Pathway

  • Open Access

    ARTICLE

    Arbuscular Mycorrhizal Fungi Improve Drought Tolerance of Quinoa Grown in Compost-Amended Soils by Altering Primary and Secondary Metabolite Levels

    Wissal Benaffari1,2,3, Fatima-Ezzahra Soussani1,2,4, Abderrahim Boutasknit1,2,5, Salma Toubali1,2,3, Abir Ben Hassine3, Hala Ben Ahmed3, Rachid Lahlali7,*, Abdelilah Meddich1,2,6,*

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.055052

    Abstract Quinoa (Chenopodium quinoa) has recently gained popularity as a pseudo-cereal cultivated in various countries due to the nutritional and antioxidant benefits of its seeds, and its capacity to persist in water-stressed environments. Our study aimed to assess the effects of native arbuscular mycorrhizal fungi (AMF) and local organic amendments on the metabolic responses and antioxidant activity of quinoa seeds under water-stressed conditions. To this end, quinoa plants were grown in soils inoculated with an indigenous mycorrhizal consortium AMF and amended with two types of compost from horse manure (HM) and green waste (GW) under two water… More >

  • Open Access

    REVIEW

    Plant Extracts as Biostimulant Agents: A Promising Strategy for Managing Environmental Stress in Sustainable Agriculture

    Mingzhao Han1, Susilawati Kasim1,*, Zhongming Yang2, Xi Deng2, Noor Baity Saidi3, Md Kamal Uddin1, Effyanti Mohd Shuib1

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.054009

    Abstract It is imperative to enhance crop yield to meet the demands of a burgeoning global population while simultaneously safeguarding the environment from adverse impacts, which is one of the dominant challenges confronting humanity in this phase of global climate change. To overcome this problem and reduce dependency on chemical fertilizer, scientists now view the implementation of biostimulant strategies as a cost-effective and environmentally friendly approach to achieving sustainable agriculture. Plant extracts are rich in bioactive phytocompounds, which can enhance plant resistance to disease, pest, and abiotic stresses (e.g., drought, salinity, and extreme temperature), and promote… More >

  • Open Access

    ARTICLE

    Salicylic Acid Improved the Growth of Soybean Seedlings by Regulating Water Status and Plant Pigments and Limiting Oxidative Injury under Salinity Stress

    Shahin Imran1,2,*, Md. Asif Mahamud3, Newton Chandra Paul1, Prosenjit Sarker4, Md. Tahjib-Ul-Arif5, Nazmul Islam5, Mohammad Saidur Rhaman6, Saleh H. Salmen7, Sulaiman Ali Alharbi7, Mohammad Javed Ansari8, Mohammed Ali Alshehri9, Akbar Hossain10,*

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.055736

    Abstract Soybean (Glycine max) is a potential legume crop, but it cannot thrive in mild salinity. Salicylic acid (SA) is a renowned plant growth hormone that improves tolerance to saline conditions. Hence, the study was performed to understand the functions of priming seeds and supplementation of SA in modulating salt tolerance in soybean seedlings. When exposed to salt stress, soybean seedlings showed considerably higher contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) while having decreased germination and growth factors, water contents, and photosynthetic pigments. The germination rate, final germination percentage, germination index, germination energy, and seed vigor index… More >

  • Open Access

    High Throughput Image Analysis between Seed Traits Opens New Breeding Avenues in Tartary Buckwheat Germplasm

    Bo Hwan Kim1,#, Sheikh Mansoor2,#, Gyung Deok Han3, Ji Eun Park4, Wook Kim1,*, Yong Suk Chung2,*

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.056439

    Abstract Recognizing the variation of genetic resources is the first step in selection. One of the most important variations in grain crops is the uniformity of seed grain weight, which can be converted into seed size. However, it has been challenging since it needs high labor costs and time to measure it on a large scale. The current study used an image analysis technique to measure the grain seed area of about 100 seeds per accession with 64 germplasm of Tartary buckwheat (Fagopyrum tataricum) to study variation among and within them. To understand the nature of variation, More >

  • Open Access

    ARTICLE

    Effect of Organic Waste and Inorganic Additives on Organic Matter Transformation and Mineral Availability in Composting Green Waste

    Abderrahim Boutasknit1,2,3, Mohamed Anli3, Rachid Lahlali4,*, Abdelilah Meddich2,3,5,*

    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.055629

    Abstract Applying organic waste and inorganic additives during composting can be an effective and easy-to-control strategy for optimizing humification, maturation, and the availability of essential mineral elements in compost. In this respect, this study aims to evaluate the effect of organic (olive-mill waste and horse manure) and inorganic (phosphogypsum) additives on the evolution of maturity indices, aromatic compounds, and nutrient availability during composting. Four mixtures [horse manure + green waste (M1), olive-mill + green waste (M2), sewage-sludge + phos-phogypsum + green waste (M3), and green waste (M4)] were carried out. Physicochemical (temperature, pH, phosphorus, nitrogen, and… More >

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