Alfaro-Corres Arnoldo Enrique^1,#, Lecona-Guzman Carlos Alberto^1,#, Gutiérrez-Miceli Federico Antonio¹, Martínez-Esteves Manuel², Silverio-Gómez María del Carmen³, Medina-Lara María de Fátima², Ruiz-Lau Nancy^4,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 629-643, 2023, DOI:10.32604/phyton.2022.023970

Abstract The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L. In order to do the establishment of cells, the formation of callus was induced; for it two auxins were evaluated: 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-mino-3,5,6-trichloropicolinic acid (picloram) at three concentrations (0.25, 0.5 and 0.75 mg L⁻¹) in three explants (leaf, root and meristems) cultured in MS semisolid medium. The callogenesis response was related to the type and section of the explant, as well as the regulator used, and a cell suspension was established using 0.5… More >

Sheila Jazmín Reyes-Zambrano^1,*, Carlos Alberto Lecona-Guzmán¹, Federico Antonio Gutiérrez-Miceli¹, Víctor Manuel Ruiz-Valdiviezo¹, Arely Anayansi Vargas-Díaz²

Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1727-1739, 2021, DOI:10.32604/phyton.2021.016171

Abstract Agave americana L. callus were exposed to different concentrations of ethyl methanesulphonate (EMS) 0, 15, 30, 45 and 60 mM and to different times of exposure (2 and 4 h). The viability and capacity of shoot formation were shown to be affected when the callus were exposed to high concentrations (30–60 mM). Only the callus exposed to 15 mM EMS presented shoot formation; the exposure time of two hours produced the largest quantity of shoots regenerated per callus (21 shoots/callus). In order to generate somaclonal variants resistant to Fusarium oxysporum, a selection pressure was applied through of a culture filtrate… More >

S. J. Reyes-Zambrano^1,†, M. L. Ramírez-Merchant^1,†, C. Arias-Castro², M. A. Rodríguez-Mendiola², C. A. Lecona-Guzmán¹, V. M. Ruíz-Valdiviezo¹, D. González-Mendoza³, F. A. Gutiérrez-Miceli^1,*

Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 277-284, 2019, DOI:10.32604/phyton.2019.06504

Abstract A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 μM) and BAP (2.68 μM) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 μM) and BAP (44 μM). The EMS induced variations in the morphometric and morphological parameters of the plantlets obtained, with… More >