LUIS A. CARO1, NATALIA SANTECCHIA1, PABLO A. MARINANGELI1, NÉSTOR R. CURVETTO1,2, LUIS F. HERNÁNDEZ1,3
BIOCELL, Vol.27, No.3, pp. 311-318, 2003, DOI:10.32604/biocell.2003.27.311
Abstract The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with… More >
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