Alexis Salazar-Navarro¹, Víctor Ruíz-Valdiviezo², José Joya-Dávila¹, Daniel González-Mendoza^1,*, Olivia Tzintzun-Camacho¹, Ulin Basilio-Cortes¹, Dagoberto Duran-Hernández¹, Henry López-Lopez¹, Onecimo Grimaldo-Juárez¹

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 641-655, 2025, DOI:10.32604/phyton.2025.062871 - 31 March 2025

Abstract The biochemical response of Coffea arabica var. Borbon to chitosan and chitosan oligosaccharides (COS) was evaluated in one-year-old plants under greenhouse conditions. COS solutions were synthesized through chemical and physical hydrolysis using acetic acid, hydrogen peroxide, and microwave irradiation. The obtained COS had an average molecular weight (Mw) of 3549.90 ± 0.33 Daltons (Da), a deacetylation degree (DD) of 76.64 ± 1.12%, and a polymerization degree (PD) of 18.91 ± 0.0018. Solutions of chitosan and COS were applied to C. arabica var. Borbon at concentrations of 0.25, 0.5, and 1 wt%. The experimental design was conducted using… More >

Ahmed Mahmoud Ismail^1,2,*, Khalid Alhudaib¹, Donato Magistà^3,4

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 679-693, 2025, DOI:10.32604/phyton.2025.062196 - 31 March 2025

Abstract Coffee wilt represents one of the most devastating diseases of Arabica coffee (Coffea arabica L.) plantations in the primary coffee-producing regions. In this study, coffee trees manifesting wilt symptoms accompanied by the defoliation and drying of the whole tree were observed in the Jazan, El Baha, Najran, and Asir regions. The purpose of this investigation was to isolate and identify the Fusarium species recovered from symptomatic coffee trees. The developed fungi were initially characterized based on their morphological features followed by molecular phylogenetic multi-locus analysis of the combined sequences of ITS, TEF1-α, RPB2, and CaM. Twenty-five isolates… More >

Alexis Salazar-Navarro¹, Victor Ruiz-Valdiviezo², Jose Joya-Dávila³, Daniel Gonzalez-Mendoza^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.5, pp. 923-949, 2024, DOI:10.32604/phyton.2024.049612 - 28 May 2024

Abstract Coffee Leaf Rust (CLR) is caused by Hemileia vastatrix in Coffea spp. It is one of the most dangerous phytopathogens for coffee plantations in terms of coffee productivity and coffee cup quality. In this review, we resume the problem of CLR in Mexico and the pathogenesis of H. vastatrix. The review abord plant-pathogen interactions which lead a compatible or incompatible interactions and result in CLR disease or resistance, respectively. The review abord Coffea spp. defense response pathways involved in H. vastatrix pathogenicity. Additionally, current measures to control H. vastatrix proliferation and germination were aborded focused on phytosanitary actions, and biological More >

Eliana Arias-Pérez¹, Carlos Alberto Lecona-Guzmán¹, Federico Antonio Gutiérrez-Miceli¹, Joaquín Adolfo Montes-Molina¹, Nancy Ruiz-Lau^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1741-1748, 2021, DOI:10.32604/phyton.2021.016004 - 28 June 2021

Abstract The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos (Coffea arabica L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L⁻¹ of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L⁻¹ of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L⁻¹ BA + 1 mg · L⁻¹ NAA + 3% (w/v) alginate, and MS + 7 mg · L⁻¹ BA + 5.7 mg · L⁻¹ indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate More >