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  • Open Access


    Analysis and Verification of the Conserved MYB Binding Element in the DFR Promoter in Compositae

    Jialei Guo1,2, Fengzhen Li2, Guomin Shi3, Weimin Zhao2, Tao He1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 343-353, 2024, DOI:10.32604/phyton.2024.047429

    Abstract Anthocyanins, ubiquitous in the Compositae family, are regulated by MYB (v-myb avian myeloblastosis viral oncogene homolog), playing an important role in anthocyanin synthesis. In this study, we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase (DFR) expression in Compositae, and validated this law in Saussurea medusa through yeast one-hybrid experiments. Our results showed that MYB and DFR underwent purification selection, DFR promoter analysis revealed the presence of MYB binding site (GAGTTGAATGG) and bHLH binding site (CANNTG) at the sense strand of 84–116 nucleotide residues from the start codon. These two motifs were separated… More >

  • Open Access


    Transcriptome Profiling of Flower Development Reveals Key Genes Mediating Yellow Formation in Tree Peony

    Yanzhao Zhang*, Chenyang Yu, Xi Lu, Yanwei Cheng

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2505-2518, 2022, DOI:10.32604/phyton.2022.021455

    Abstract Tree peony cultivars with yellow flowers are rare and valuable, but the molecular mechanism of pigment accumulation is not clear. In this study, the petal transcriptome of three developmental stages were sequenced to determine the differentially expressed genes (DEGs) regulating yellow flowers color. The results showed that 10,842 and 12,022 DEGs were screened in stage 1 vs. stage 2 and in stage 2 vs. stage 3, respectively. Through analysis of flavonoid structural genes (FSGs), we found that the transcription level of DFR was very low in the three developmental stages. In a small group of cultivars, the DFR transcription level… More >

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