Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (2)
  • Open Access


    Selection and Validation of Reference Genes for Normalization of RT-qPCR Analysis in Developing or Abiotic-Stressed Tissues of Loquat (Eriobotrya japonica)

    Shoukai Lin1,2,#, Shichang Xu1,#, Liyan Huang1, Fuxiang Qiu1, Yihong Zheng1, Qionghao Liu1, Shiwei Ma1,2, Bisha Wu1,2, Jincheng Wu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1185-1201, 2023, DOI:10.32604/phyton.2023.026752

    Abstract Loquat (Eriobotrya japonica Lindl.) is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components. Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression. In this study, eight candidate reference genes were selected from our previously published RNA-seq data, and primers for each candidate reference gene were designed and evaluated. The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples, including 12 subgroups of developing or abiotic-stressed tissues. Different combinations of stable… More >

  • Open Access


    Seed Germination Traits of Loquat (Eriobotrya japonica Lindl.) as Affected by Various Pre-Sowing Treatments (Cutting of Cotyledons, Removal of Perisperm, Moist Chilling and/or Exogenous Application of Gibberellin)

    Thiresia-Teresa Tzatzani1, Evangelia Basdeki2, Evangelia-Vasiliki Ladikou3, Marios-Ioannis N. Sotiras3, Georgios Panagiotakis4, Sryridon Lionakis4, Ioannis E. Papadakis3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 645-656, 2020, DOI:10.32604/phyton.2020.010532

    Abstract The purpose of this study was to investigate the effects of various presowing treatments on the germinability (final germination percentage) and germination rate of loquat seeds in order to increase seedling production in nurseries (applied research) as well as provide answers for important physiological issues related to loquat seeds and their seed coat (basic research). Three experiments were carried out with various pre-sowing treatments. These treatments included full or partial removal of seed coat (perisperm), partial cutting of cotyledons as well as moist chilling at 5°C for 13 days and/or soaking the seeds in water… More >

Displaying 1-10 on page 1 of 2. Per Page