KE JIA¹, FU-QING TAN², SHUANG-LI HAO^1,*, WAN-XI YANG^1,*

BIOCELL, Vol.46, No.11, pp. 2399-2413, 2022, DOI:10.32604/biocell.2022.022015

Abstract The SRY-related high mobility group (HMG) box (SOX) transcription factors participate in many physiological processes of animal growth, development, and reproduction and are related to spermatogenesis in many species. However, the relationship between SOX and spermatogenesis in Eriocheir sinensis is rarely reported. Here, we studied the role of Es-SOX8 in the spermatogenesis of E. sinensis and its possible regulation mechanism. Immunofluorescence results demonstrated Es-SOX8 signal in both cytoplasm and nucleus of spermatogonia, spermatocytes as well as spermatids, but not in mature spermatozoa. Hematoxylin and eosin staining showed a significant increase in the number of spermatozoa with abnormal nuclear morphology   … More >

SHUANG-LI HAO, FEI-DA NI, BANG-HONG WEI, ZHEN-FANG LI, TONG YANG, WAN-XI YANG^*

BIOCELL, Vol.45, No.4, pp. 971-984, 2021, DOI:10.32604/biocell.2021.015178

Abstract Spermatogenesis is a complicated and highly regulated male gamete differentiation process that begins with the proliferation and differentiation of spermatogonia to the release of the mature spermatozoa. The autophagy-related gene atg7 has been reported as closely related to spermatogenesis and communication of Sertoli cell-germ cells in mice, including acrosome biogenesis, sperm flagellum development, and ectoplasmic specialization assembly. However, the function of es-ATG7 and its molecular regulatory mechanism during spermatogenesis in Crustacea remain largely unknown. Here, we cloned and identified es-atg7 from the testes of the Chinese mitten crabs Eriocheir sinensis and found that the expression of es-atg7 was relatively high… More >