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    ARTICLE

    Cloning and Bioinformatics Analysis of the GlROP6 gene in Glehnia littoralis

    Min Yan1, Han Wang1, Shaohua Liu1, Ye Xu1, Zizhuo Nie1, Yifeng Zhou2, Li Li2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1293-1300, 2021, DOI:10.32604/phyton.2021.015601

    Abstract Rho-related GTPase from plants (ROP) proteins play an essential role in plant stress resistance. In this study, the full-length GlROP6 gene was cloned based on G. littoralis transcriptome sequencing data acquired in response to salt stress. The protein sequence, conserved domains, secondary structure, three-dimensional structure, phylogenetic relationships, and expression pattern of the GlROP6 gene were systematically analysed. Our results showed that the full-length GlROP6 gene had an open reading frame of 606 bp, which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06. Amino acid sequence analyses indicated that… More >

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