Si ran Wang#, Jia Yan#, Bu er Ha, Yu’e Bai*
Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 595-604, 2021, DOI:10.32604/phyton.2021.013693
Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering
technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established
an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant
growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL−1
6-BA and 0.5 mgL−1
1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots proliferation
coefficient… More >
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