Yujia Leng^1,#, Shuilian Wang^1,2,3,#, Ruoan Wang^2,#, Tao Tao¹, Shuwen Jia^1,2, Tao Song^2,3, Lina Xu^1,2, Xiuling Cai^1,2, Sukui Jin^1,2,*, Jiping Gao^1,2,4,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 943-958, 2022, DOI:10.32604/phyton.2022.018707

Abstract Grain size traits are critical agronomic traits which directly determine grain yield, but the genetic bases of these traits are still not well understood. In this study, a total of 154 chromosome segment substitution lines (CSSLs) population derived from a cross between a japonica variety Koshihikari and an indica variety Nona Bokra was used to investigate grain length (GL), grain width (GW), length-width ratio (LWR), grain perimeter (GP), grain area (GA), and thousand grain weight (TGW) under four environments. QTL mapping analysis of six grain size traits was performed by QTL IciMapping 4.2 with an inclusive composite interval mapping (ICIM)… More >

Wuzhong Yin^#, Hongxia Yang^#, Yantong Wang, Ping Feng, Yao Deng, Yang Liu, Danyang Chen, Yijie Ban, Weichi Liu, Guanghua He^*, Nan Wang^*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 727-744, 2022, DOI:10.32604/phyton.2022.017811

Abstract The mitogen-activated protein kinase (MAPK) cascade is important in stress signal transduction and plant development. In the present study, we identified a rice (Oryza sativa L.) mutant with reduced fertility, Oryza sativa mitogen-activated protein kinase 6 (osmapk6), which harbored a mutated MAPK gene. Scanning and transmission electron microscopy, quantitative RT-PCR analysis, TUNEL assays, RNA in situ hybridization, longitudinal and transverse histological sectioning, and map-based cloning were performed to characterize the osmapk6 mutant. The gene OsMAPK6 was expressed throughout the plant but predominantly in the microspore mother cells, tapetal cells, and microspores in the anther sac. Compared with the wild type,… More >

JAVARIA AFZAL^1,2,#, XIUKANG WANG^3,*, MUHAMMAD HAMZAH SALEEM^4,#, XUECHENG SUN¹, SHAHID HUSSAIN⁵, IMRAN KHAN⁶, MUHAMMAD SHOAIB RANA¹, SHAKEEL AHMED⁷, SAMRAH AFZAL AWAN⁶, SAJID FIAZ⁸, OMAR AZIZ⁹, KASHIF ALI KUBAR¹⁰, SHAFAQAT ALI^11,12, CHENGXIAO HU^1,*

BIOCELL, Vol.45, No.6, pp. 1631-1649, 2021, DOI:10.32604/biocell.2021.014934

Abstract Soil contamination with toxic heavy metals [such as cadmium (Cd)] is becoming a serious global problem due to rapid development of social economy. Iron (Fe), being an important element, has been found effective in enhancing plant tolerance against biotic and abiotic stresses. The present study investigated the extent to which different levels of Ferrous sulphate (FeSO₄) modulated the Cd tolerance of rice (Oryza sativa L.), when maintained in artificially Cd spiked regimes. A pot experiment was conducted under controlled conditions for 146 days, by using natural soil, mixed with different levels of CdCl₂ [0 (no Cd), 0.5 and 1 mg/kg]… More >

Shaoyi Ruan^1,2,3,#, Haowen Luo^1,2,3,#, Xiaoyan Zeng^1,2,3,#, Runhao Wen^1,2,3, Feida Wu^1,2,3, Xiangru Tang^1,2.*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1273-1283, 2021, DOI:10.32604/phyton.2021.015610

Abstract Vermicompost is an organic fertilizer contains multiple nutrient elements. However, the application of vermicompost in fragrant rice production is rarely reported. In order to study the effects of vermicompost application on fragrant rice (Oryza sativa L.) seedling performances, present study was conducted with two fragrant rice cultivars and four vermicompost rate treatments (0 (CK), 2.5 (Wo1), 5.0 (Wo2) and 10.0 (Wo3) g kg⁻¹ ). The results showed that vermicompost treatments significantly increased dry weight of fragrant rice seedling by 8.31–32.56% compared with CK. 21.10–59.13% higher net photosynthetic rates and 10.66–59.16% higher chlorophyll contents (chlorophyll a, chlorophyll b and total chlorophyll)… More >

Linjun Cai^1,#, Junhua Liu^2,#, Han Yun¹, Dan Du¹, Xiaolong Zhong¹, Zhenlin Yang¹, Xianchun Sang¹, Changwei Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1103-1117, 2021, DOI:10.32604/phyton.2021.015532

Abstract Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis, photomorphogenesis, hormone physiology and disease resistance. In this study, the genetically stable yellow-green leaf mutant ygl16 was identified from mutated “Xinong 1B”. Compared with the wild type, the pigment concentration and photosynthetic capacity of the ygl16 decreased significantly. The ultrastructural observation showed that the distribution of thylakoid lamellae was irregular in ygl16 chloroplasts, and the grana and matrix lamellae were blurred and loose in varied degrees, and the chloroplast structure was disordered, while the osmiophilic corpuscles increased. The results of the genetic analysis… More >

Gang Wei^#, Hongxia Yang^#, Jingwen Wu, Zixian Xiong, Nan Wang^*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 803-813, 2021, DOI:10.32604/phyton.2021.014505

Abstract There is a positive correlation between fertility and yield, and the decrease of fertility is bound to a greatly reduced crop yield. Male sterile mutants can be used in hybrid rice. Therefore, rice male sterility has an important value in research and application, and the study of related mutants is also very vital. The mutant ms10 (male sterile 10) reported in this study was induced by ethyl methane sulfonate (EMS) in the indica maintainer line Xinong 1B. There was no significant difference between the ms10 and wild type in the vegetative growth stage. However, in the reproductive growth stage, ms10… More >

Xiaohong Yin, Zui Tao, Jiana Chen, Fangbo Cao, Min Huang^*, Yingbin Zou

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 415-422, 2020, DOI:10.32604/phyton.2020.08962

Abstract It has been shown that adding biochar to soil can improve nitrogen (N) uptake and utilization in rice (Oryza sativa L.). However, there is a lack of research on the physiological alterations of rice as a result of the changes in nitrogen uptake due to the addition of biochar. This study conducted field experiments in 2015 and 2016 with the goal of testing the hypothesis that the application of biochar would enhance radiation use efficiency (RUE) of rice by improving the plant’s ability to take in and utilize nitrogen. Our results demonstrated that the application of biochar (20 t ha⁻¹… More >

R. MEDINA, M. FALOCI, M.A. MARASSI, AND L.A. MROGINSKI.

BIOCELL, Vol.28, No.1, pp. 13-20, 2004, DOI:10.32604/biocell.2004.28.013

Abstract An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l^-1. Shoot clumps were multiplied in MS liquid medium containing BAP 5 mg l^-1.… More >

Liu M^1,2, Z-W Liang^*1,2, L-H Huang^1,2, M-M Wang^1,2, H-Y Yang^1,2

Phyton-International Journal of Experimental Botany, Vol.85, pp. 162-168, 2016, DOI:10.32604/phyton.2016.85.162

Abstract Saline-sodic stress is a major abiotic constraint responsible for rice (Oryza sativa L.) yield reductions in Northeast China. The rice root system is crucial for yield development, and it is usually recognized as the key for improving future crop productivity. However, most of the saline-sodic soils in these areas contain high levels of soluble Na₂CO₃ and NaHCO₃, which results in a high pH (>8.5), clay dispersion, soil swelling, and overall poor soil physical properties. Isolation, washing and measurement of the rice crown roots is highly time-demanding in this kind of soil. Our aim was to explore whether differences in shoot… More >