Anna Stepanova1,*, Maria Malunova1, Svetlana Salamaikina1, Renat Selimov2, Aleksandra Solov’eva1
Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 543-552, 2021, DOI:10.32604/phyton.2021.013223
Abstract Rhodiola quadrifida is a rare mountain medicinal plant whose root
extracts are used in traditional Chinese medicine as a hemostatic, antitussive,
and tonic in the treatment of gynecological diseases. The aim of the study was
to obtain R. quadrifida cultures at different degrees of differentiation in vitro
and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and
rolC genes was proven by polymerase chain reaction. The obtained roots were
cultivated in Murashige-Skoog medium (MS). Calluses… More >
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