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  • Open Access

    ARTICLE

    Metabolomic and Transcriptomic Insights into Enhanced Paclitaxel Biosynthesis in Cultivated Taxus cuspidata

    Dandan Wang*, Jiaxin Chen, Yanwen Zhang

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1137-1158, 2025, DOI:10.32604/phyton.2025.063894 - 30 April 2025

    Abstract Taxus cuspidata, a rare species of the Taxus genus, and its wild resources are under severe threat. The development of cultivated species has become an important strategy to replace wild species. The objective of this work was to elucidate the differences in secondary metabolite accumulation, particularly in the paclitaxel biosynthesis pathway, between wild and cultivated species. This study employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) and RNA sequencing (RNA-Seq) technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T. cuspidata. The results showed that the content of paclitaxel in cultivated species was significantly higher… More >

  • Open Access

    ARTICLE

    Comparative Study of Genetic Structure and Genetic Diversity between Wild and Cultivated Populations of Taxus cuspidata, Northeast China

    Dandan Wang, Xiaohong Li, Yanwen Zhang*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 355-369, 2024, DOI:10.32604/phyton.2024.047183 - 27 February 2024

    Abstract Taxus cuspidata is a rare plant with important medicinal and ornamental value. Aiming at the obvious differences between wild and cultivated populations of T. cuspidata from Northeast China, a total of 61 samples, that is, 33 wild yews and 28 cultivated yews were used to analyze the differences and correlations of the kinship, genetic diversity, and genetic structure between them by specific length amplified fragment sequencing (SLAF-seq). Finally, 470725 polymorphic SLAF tags and 58622 valid SNP markers were obtained. Phylogenetic analysis showed that 61 samples were classified into 2 clusters: wild populations and cultivated populations, and some… More >

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