Huanli Wang, Lingjun Yan, Xi Huang, Zhongwei Wang, Yuanhao Yue, Shijie Tang*
Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2191-2210, 2022, DOI:10.32604/phyton.2022.020735
Abstract Quantitative real-time polymerase chain reaction (qRT-PCR) is a rapid and effective approach toward detecting the expression patterns of target genes. The selection of a stable reference gene under specific test condition is essential for expressing levels of target genes accurately. Tilia miqueliana, considered endangered, is a prominent native ornamental and honey tree in East China. No study has evaluated the optimal endogenous reference gene for qRT-PCR analysis in T. miqueliana systematically. In this study, fifteen commonly used reference genes were selected as candidate genes, and the stabilities of their expressions were assessed using four algorithms (GeNorm, NormFiner, BestKeeper, and DeltaCt)… More >
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