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    Molecular differentiation of Xoconostle (Opuntia spp.) using AFLP molecular markers

    García-Zambrano EA1, MC Ojeda-Zacarías1, JG Gutiérrez-Soto1, SR Sinagawa-García1, A Gutiérrez-Díez1, J Martínez-de la Cerda1, JA Pedroza-Flores1, C Gallegos-Vázquez2, JI García-Zambrano3

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087

    Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that the DNA concentrations ranged… More >

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