YANG CAO1,#, YINING LIU1,#, YANG YU1, XIAOFEI GUO1, XIUXIU WANG1, WENYA MA1, HANJING LI2, ZHONGYU REN2, XINLU GAO2, SIJIA LI2, HAOYU JI2, HONGYANG CHEN2, HONG YAN2, YANAN TIAN2, XIN WANG2, BENZHI CAI1,2,*
BIOCELL, Vol.47, No.6, pp. 1407-1416, 2023, DOI:10.32604/biocell.2023.029080
Abstract Background: Cardiomyocytes derived from human embryonic stem cells (hESCs) are regulated by complex and stringent gene networks during differentiation. Long non-coding RNAs (lncRNAs) exert critical epigenetic regulatory functions in multiple differentiation processes. However, the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully elucidated. Here, we identified the key roles of ZFAS1 (lncRNA zinc finger antisense 1) in the differentiation of cardiomyocytes from hESCs. Methods: A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method, and the number of beating hESCs-derived cardiomyocytes was calculated. Gene expression was analyzed by… More >
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