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    ARTICLE

    The actin filament network associated to Sertoli cell ectoplasmic specializations

    JUAN CARLOS CAVICCHIA1*, MABEL FÓSCOLO1 , JORGE IBAÑEZ1, CHRISTOPHER LILLIG2, FRANCISCO CAPANI3

    BIOCELL, Vol.35, No.3, pp. 81-90, 2011, DOI:10.32604/biocell.2011.35.081

    Abstract Junctional devices in Sertoli cells conform the blood-testis barrier and play a key role in maturation and differentiation of germ cells. The spacial distribution of ectoplasmic specializations of Sertoli cells was studied by β-actin immunolabelling, using laser confocal and transmission electron microscopy. For confocal microscopy, β-actin immunolabelling of ectoplasmic specializations was studied over the background of either prosaposin or glutaredoxin immunolabelling of the Sertoli cytoplasm. Labelling was found near the basal lamina, surrounding early spermatocytes (presumably in leptotene-zygotene) or at one of two levels in the seminiferous epithelium: (1) around deep infoldings of the Sertoli cell cytoplasm, in tubular stages… More >

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