Manoochehr MESSRIPOUR1, Azadeh MESRIPOUR2
BIOCELL, Vol.43, No.1, pp. 37-40, 2019, DOI:10.32604/biocell.2019.05865
Abstract DNA damage and the enzyme poly (ADP-ribose) polymerase (PARP) associated with the pathogenesis
of numerous age-related neurodegenerative disorders. Astrocytes play crucial roles in both support metabolic
functions and cell viability of the brain. PARP regulates DNA damage and repair in the brain cells. In this study PARP
activity and DNA strand break were investigated in the astrocytes isolated from young and aged rat brain. Three and
30-month-old rats were killed by decapitation and brains were removed onto an ice cooled glass plate. Astrocytes were
isolated by sucrose density gradient centrifugation and glutamine synthetase (GS) served as a marker of the… More >
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