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  • Open Access

    ARTICLE

    Chromosomal location of four genes encoding Class III peroxidases in wheat

    Simonetti E1, E Alba2, A Delibes2

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 139-142, 2012, DOI:10.32604/phyton.2012.81.139

    Abstract In a previous work, deduced amino acid sequences from twenty wheat peroxidase genes were assigned to seven groups designated as TaPrx108 to TaPrx114. Some of these apoplastic peroxidases have previously shown to play different roles in the plant defense responses to infection by the cereal cyst nematode Heterodera avenae. In the present study, PCR marker analysis using Sears’s aneuploid wheat lines cv. ‘Chinese Spring’ was used to locate four genes encoding peroxidase isozymes. The TaPrx111-A, TaPrx112-D and TaPrx113-F genes were located on the short arm of chromosome 2B and the TaPrx109-C on the long arm of chromosome 1B. More >

  • Open Access

    ARTICLE

    Construction and characterization of a metagenomic DNA library from the rhizosphere of wheat (Triticum aestivum)

    Hernández-León R1, M Martínez-Trujillo2, E Valencia-Cantero1, G Santoyo1

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 133-137, 2012, DOI:10.32604/phyton.2012.81.133

    Abstract Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), More >

  • Open Access

    ARTICLE

    Bacterial diversity associated with the rhizosphere of wheat plants (Triticum aestivum): Toward a metagenomic analysis

    Velázquez-Sepúlveda I, MC Orozco-Mosqueda, CM Prieto-Barajas, G Santoyo

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 81-87, 2012, DOI:10.32604/phyton.2012.81.081

    Abstract Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since More >

  • Open Access

    ARTICLE

    Selection of double purpose wheat genotypes. Vernalization requirements

    Morant AE, HD Merchán, EE Lutz

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 5-8, 2011, DOI:10.32604/phyton.2011.80.005

    Abstract This study aimed to characterize wheat cultivars for their chilling requirements for differentiation and their relationship to flowering time, to establish a dual purpose ideotype. Seeds of eight genotypes (cultivars and advanced lines) of bread wheat were exposed to constant temperature (4 °C) in germination chambers for 8, 6, 4 and 2 weeks. A control treatment was kept at room temperature. Four replicates of each treatment were placed in the greenhouse in 250 cc pots containing 4 seedlings each, with irrigation and natural photoperiod, following a randomized complete block design. The greenhouse temperature was constant… More >

  • Open Access

    ARTICLE

    The Global Nonlinear Galerkin Method for the Solution of von Karman Nonlinear Plate Equations: An Optimal & Faster Iterative Method for the Direct Solution of Nonlinear Algebraic Equations F(x) = 0, using x· = λ[αF + (1 - α)BTF]

    Hong-Hua Dai1,2, Jeom Kee Paik3, S. N. Atluri2

    CMC-Computers, Materials & Continua, Vol.23, No.2, pp. 155-186, 2011, DOI:10.3970/cmc.2011.023.155

    Abstract The application of the Galerkin method, using global trial functions which satisfy the boundary conditions, to nonlinear partial differential equations such as those in the von Karman nonlinear plate theory, is well-known. Such an approach using trial function expansions involving multiple basis functions, leads to a highly coupled system of nonlinear algebraic equations (NAEs). The derivation of such a system of NAEs and their direct solutions have hitherto been considered to be formidable tasks. Thus, research in the last 40 years has been focused mainly on the use of local trial functions and the Galerkin… More >

  • Open Access

    ARTICLE

    The Global Nonlinear Galerkin Method for the Analysis of Elastic Large Deflections of Plates under Combined Loads: A Scalar Homotopy Method for the Direct Solution of Nonlinear Algebraic Equations

    Hong-Hua Dai1,2, Jeom Kee Paik3, Satya N. Atluri2

    CMC-Computers, Materials & Continua, Vol.23, No.1, pp. 69-100, 2011, DOI:10.3970/cmc.2011.023.069

    Abstract In this paper, the global nonlinear Galerkin method is used to perform an accurate and efficient analysis of the large deflection behavior of a simply-supported rectangular plate under combined loads. Through applying the Galerkin method to the governing nonlinear partial differential equations (PDEs) of the plate, we derive a system of coupled third order nonlinear algebraic equations (NAEs). However, the resultant system of NAEs is thought to be hard to tackle because one has to find the one physical solution from among the possible multiple solutions. Therefore, a suitable initial guess is required to lead… More >

  • Open Access

    ARTICLE

    Toxicity and accumulation of arsenic in wheat (Triticum aestivum L.) varieties of China

    Zhang1 WD, DS Liu2, JC Tian1*, FL He1

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 147-154, 2009, DOI:10.32604/phyton.2009.78.147

    Abstract Soil arsenic contamination becomes a potential agricultural and environmental hazard worldwide, and has been a serious problem for safe food production. A field experiment on soil contamination was conducted on four wheat varieties (Jimai, Gaoyou, Weimai and Wennong) in Eastern China, using 50 or 100 mg arsenic/kg soil. Biomass production and yield components were determined and arsenic concentrations were measured in plant tissues. Differential arsenic effects on wheat varieties were determined at maturity. Results showed that addition of arsenic significantly (p<0.05) reduced root, stem and spike dry weight and yield components, which resulted in the… More >

  • Open Access

    ARTICLE

    Mixture of wheat varieties for dual purpose

    Lutz EE, HD Merchán, AE Morant

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 217-223, 2008, DOI:10.32604/phyton.2008.77.217

    Abstract Forage and grain production of an early sown, long-cycle wheat variety (ProINTA Super) was compared to that in its association with a short-cycle wheat variety (Buck Pronto), both varieties exposed to one or two defoliations. The purpose was producing an earlier and greater amount of dry matter in a dual-purpose wheat. Research was conducted in the Asociación de Cooperativas Argentinas experimental field (38º 36´ S, 61º 58´ W, 122 masl), 15 km SW from Cabildo (Bs. As), in the semi-arid V South wheat subregion. Experimental plots, 7 rows 4m long each and 0,20 m apart… More >

  • Open Access

    ARTICLE

    Production of a double purpose wheat defoliated at different developmental stages of the growth apex

    Merchán HD, EE Lutz, AE Morant

    Phyton-International Journal of Experimental Botany, Vol.76, pp. 133-142, 2007, DOI:10.32604/phyton.2007.76.133

    Abstract In mixed systems (beef and grain), double purpose wheat is an alternative to reduce competition between the winter cereals for grazing and wheat for harvesting. In order to optimize grass production and avoid grain yield decreases, it is necessary to use varieties with long vegetative periods and the last defoliation should be made before the apex changes to the reproductive developmental stage. A study was conducted in the experimental field of the Asociación de Cooperativas Argentinas in Cabildo (38º 36' S, 61º 58' W) to evaluate grass and grain production of the facultative wheat variety (TriticumMore >

  • Open Access

    ARTICLE

    Forage characteristics for double purpose wheats

    Morant AE, HD Merchán, EE Lutz

    Phyton-International Journal of Experimental Botany, Vol.76, pp. 95-102, 2007, DOI:10.32604/phyton.2007.76.095

    Abstract Length of the vegetative period and forage production were evaluated in ten commercial, long term cycle wheat varieties: Buck Charrúa, Cooperación Nanihué, Buck Cacique, Cooperación Maipún, Buck Catriel, ProINTA Pigüé, Buck Poncho, ProINTA Pincén, ProINTA Super and Cooperación Liquén. The study was conducted in the experimental field of the Departmento de Agronomia, Universidad Nacional del Sur, Bahía Blanca, Argentina, under dry land conditions. A completely Randomized Block Design with five replicates was used. Seeding was effected on 9 April 1996. From the time the plant had three leaves to the Double Ridge (DA) apex stage,… More >

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