Open Access

ARTICLE

Bacterial diversity associated with the rhizosphere of wheat plants (Triticum aestivum): Toward a metagenomic analysis

Velázquez-Sepúlveda I, MC Orozco-Mosqueda, CM Prieto-Barajas, G Santoyo

Instituto de Investigaciones Químico Biológicas. Universidad Michoacana de San Nicolás de Hidalgo, México.
Address Correspondence to: Gustavo Santoyo. Instituto de Investigaciones Químico Biológicas. Universidad Michoacana de San Nicolás de Hidalgo. Ciudad universitaria edif. A1’, 58030. Morelia, Michoacán, México, e-mail: gustavo_santoyo@yahoo.com ; gsantoyo@umich.mx

Phyton-International Journal of Experimental Botany 2012, 81(all), 81-87. https://doi.org/10.32604/phyton.2012.81.081

Abstract

Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they corresponded to 40% of the whole ribosomal library. Phylogenetic analysis showed that most of the ribosomal sequences are grouped into clades that belong to common rhizospheric or bulk-soil bacteria. To determine whether the sample is significantly diverse, a Shannon-Wiener test was performed, resulting in a rate of 3.8 bits per individual. Our results suggest that the rhizosphere of wheat plants is highly diverse and results an excellent candidate for metagenomic analysis.

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