S. Deguchi1,2, T. Ohashi2, M. Sato2
Molecular & Cellular Biomechanics, Vol.2, No.3, pp. 125-134, 2005, DOI:10.3970/mcb.2005.002.125
Abstract Actin bundles in vascular endothelial cells (ECs) play a critical role in transmitting intracellular forces between separate focal adhesion sites. However, quantitative descriptions of tension level in single actin bundles in a physiological condition are still poorly studied. Here, we evaluated magnitude of preexisting tension in a single actin bundle of ECs on the basis of measurements of its preexisting stretching strain and tensile properties. Cultured ECs expressing fluorescently-labeled actin were treated with detergents to extract acin bundles. One end of an actin bundle was then dislodged from the substrate by using a microneedle, resulting in a shortening of the… More >
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