Meghan H. Hoskins*, Cheng Dong∗,†
Molecular & Cellular Biomechanics, Vol.3, No.2, pp. 79-88, 2006, DOI:10.3970/mcb.2006.003.079
Abstract It has been determined previously that polymorphonuclear leukocytes, or PMNs, can facilitate melanoma cell extravasation through the endothelium under shear conditions [1,2]. The interactions between melanoma cells and PMNs are mediated by the β2-integrins expressed by PMNs and intercellular adhesion molecules (ICAM-1) expressed on melanoma cells. In this study, the kinetics of these interactions was studied using a parallel plate flow chamber. The dissociation rates were calculated under low force conditions for ICAM-1 interactions with both β2-integrins, LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18), together and separately by using functional blocking antibodies on PMNs. The kinetics of PMNs stimulated with IL-8 was… More >
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