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    ARTICLE

    RNA-sequencing indicates high hemocyanin expression as a key strategy for cold adaptation in the Antarctic amphipod Eusirus cf. giganteus clade g3

    SAMUELE GRECO1,#, ELISA D’AGOSTINO2,#, CHIARA MANFRIN1, ANASTASIA SERENA GAETANO1, GAEL FURLANIS1, FRANCESCA CAPANNI1, GIANFRANCO SANTOVITO2, PAOLO EDOMI1, PIERO GIULIO GIULIANINI1, MARCO GERDOL1,*

    BIOCELL, Vol.45, No.6, pp. 1611-1619, 2021, DOI:10.32604/biocell.2021.016121

    Abstract We here report the de novo transcriptome assembly and functional annotation of Eusirus cf. giganteus clade g3, providing the first database of expressed sequences from this giant Antarctic amphipod. RNA-sequencing, carried out on the whole body of a single juvenile individual likely undergoing molting, revealed the dominant expression of hemocyanins. The mRNAs encoding these oxygen-binding proteins cumulatively accounted for about 40% of the total transcriptional effort, highlighting the key biological importance of high hemocyanin production in this Antarctic amphipod species. We speculate that this observation may mirror a strategy previously described in Antarctic cephalopods, which compensates for the decreased ability… More >

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