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  • Open Access


    Analysis and Verification of the Conserved MYB Binding Element in the DFR Promoter in Compositae

    Jialei Guo1,2, Fengzhen Li2, Guomin Shi3, Weimin Zhao2, Tao He1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 343-353, 2024, DOI:10.32604/phyton.2024.047429

    Abstract Anthocyanins, ubiquitous in the Compositae family, are regulated by MYB (v-myb avian myeloblastosis viral oncogene homolog), playing an important role in anthocyanin synthesis. In this study, we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase (DFR) expression in Compositae, and validated this law in Saussurea medusa through yeast one-hybrid experiments. Our results showed that MYB and DFR underwent purification selection, DFR promoter analysis revealed the presence of MYB binding site (GAGTTGAATGG) and bHLH binding site (CANNTG) at the sense strand of 84–116 nucleotide residues from the start codon. These two motifs were separated… More >

  • Open Access


    Smart Colorimetric Corn Starch Films Combined with Anthocyanin-Loaded Glutenin-Carboxymethyl Chitosan Nanocomplexes for Freshness Monitoring of Chilled Pork

    Juan Yan1, Wenchao Li1, Xianfang Zhang1, Shisheng Liu2,*

    Journal of Renewable Materials, Vol.12, No.1, pp. 71-87, 2024, DOI:10.32604/jrm.2023.028927

    Abstract In this study, intelligent, pH-responsive colorimetric films were prepared by encapsulating anthocyanins in nanocomplexes prepared from glutenin and carboxymethyl chitosan. These nanocomplexes were added to a corn starch matrix and used in the freshness monitoring of chilled pork. The effects of anthocyanin-loaded nanocomplexes on the physical, structural, and functional characteristics of the films were investigated. The addition of anthocyanin-loaded nanocomplexes increased the tensile strength, elongation at break, hydrophobicity, and light transmittance of the films while decreasing their water vapor permeability. This is because new hydrogen bonds are formed between the film components, resulting in a more homogeneous and dense structure.… More >

  • Open Access


    Genome-Wide Analysis of the F3′5′H Gene Family in Blueberry (Vaccinium corymbosum L.) Provides Insights into the Regulation of Anthocyanin Biosynthesis

    Xiaolan Guo1,2, Jinbin Hu3, Shimei Yang4, Delu Wang2,*, Jianbing Wang1

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2683-2697, 2023, DOI:10.32604/phyton.2023.029820

    Abstract The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis, abiotic stress, and hormone signaling. In this study, 14 F3′5′H genes were identified from the blueberry genome. The chromosomal distribution, physicochemical properties, F3′5′H domain, conserved motifs, cis-acting elements, and intron/exon compositions were analyzed. The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites. The results of qRT-PCR showed that VcF3′5′Hs (especially VcF3′5′H4) were highly expressed at the ripening stage. Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum. Co-expression analysis showed that the VcF3′5′H gene family… More >

  • Open Access


    Thermally and UV Stable Natural Dyes with Potential Use in Efficient Photoelectrochemical Devices

    Mario Alejandro Mejía Escobar*, Franklin Jaramillo*

    Journal of Renewable Materials, Vol.3, No.4, pp. 302-317, 2015, DOI:10.7569/JRM.2014.634121

    Abstract Energy alternatives have been one of the most important research focal areas of the last decade due to the imminent lack of fossil fuels. This motivation has allowed the evolution of the materials science field in areas such as electrochemistry, where high availability, low cost and negligible environmental impact are the desired principal qualities. For this reason, as an example of a photoelectrochemical device, we have proposed the use of natural dyes from 37 agro-industrial wastes in dye-sensitized solar cells. We obtained dyes from red cabbage, radish and roselle wastes with a good stability for eight hours with a constant… More >

  • Open Access


    Natural Dyes Extraction, Stability and Application to Dye-Sensitized Solar Cells

    Mario Alejandro Mejía Escobar*, Frankin Jaramillo*

    Journal of Renewable Materials, Vol.3, No.4, pp. 281-291, 2015, DOI:10.7569/JRM.2014.634142

    Abstract In recent years, the development of new materials has contributed markedly to improving the efficiency of dye-sensitized solar cells (DSSCs). Mesoporous semiconductors, solid electrolyte, non-conventional catalysts and synthetic dyes without ruthenium have been the focus of study. With respect to the last, lanthanides and porphyrins have presented good properties, but these elements have the same disadvantage as rutheniumbased dyes; being complicated to synthesize and very expensive. These limitations have stimulated much research in the use of natural dyes as sensitizers due to their exceptional photoelectochemical properties and low cost. Furthermore, they are regarded as renewable and environmentally friendly materials. For… More >

  • Open Access


    Transcriptomic and Physiological Analyses of Pigment Accumulation in Eucommia ulmoides ‘Hongye’

    Mengjiao Chen1,2, Jinhui Zhai3, Jiajia Zhang1,4,5,6, Hui Li1,2, Xinjiang Niu1,2, Yaxin Liu1,2, Yue Ren1,2, Hongyan Du1,4,5,6, Jingle Zhu1,4,5,6,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 1027-1044, 2022, DOI:10.32604/phyton.2022.019106

    Abstract Eucommia ulmoides ‘Hongye’ is a new ornamental variety of E. ulmoides with excellent red or purple foliage. We found that E. ulmoides ‘Hongye’ exhibited a gradual change from green to red colour under light conditions. However, the colouring mechanism in the leaves of E. ulmoides ‘Hongye’ remains unclear. In this study, we compared the pigment content and leaf colour index of E. ulmoides ‘Hongye’ at five stages with those of E. ulmoides ‘Xiaoye’, which was used as the control variety. The transcriptome sequencing data of the first-period (H1, green) and fifth-period (H5, red) leaves were also analysed and compared. The… More >

  • Open Access


    Diversity Analysis of Chlorophyll, Flavonoid, Anthocyanin, and Nitrogen Balance Index of Tea Based on Dualex

    Yifu Liu1,2, Jiazhen Wang1,2,*, Yao Xiao1, Xiongwen Shi1, Yixia Zeng1

    Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1549-1558, 2021, DOI:10.32604/phyton.2021.015557

    Abstract The chemical compositions of tea (Camellia sinensis) are affected by numerous factors, such as cultivar, climate, leaf position, and cultivation pattern. However, under the same conditions, the chemical compositions are mainly determined by varieties. Therefore, we investigated the genetic diversity of chemical compositions of tea tree resources in China to screen excellent germplasm resources. Three chemical compositions index (including chlorophyll index, flavonoid index, and anthocyanin index) and the nitrogen balance index of tea leaves were measured in 102 tea germplasms planted in Chinese Tea Plants Improved Variety Germplasm Resources Nursery (CTPIVGRN) by Dualex on April 15 2019. Results showed that… More >

  • Open Access


    Comparative Metabolomics Analysis between Red- and White-Flowered Common Buckwheat Cultivars

    Jiao Deng1,2,#, Fen Dong3,#, Chaoxin Wu1, Jiali Zhao1, Hongyou Li1, Juan Huang1, Taoxiong Shi1, Ziye Meng1, Fang Cai1, Qingfu Chen1,*, Pingfang Yang2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 859-870, 2021, DOI:10.32604/phyton.2021.014625

    Abstract Common buckwheat (Fagopyrum esculentum), a specialty crop in southwest China, is not only used as a supplement to primary grain crops but also to produce beverages, such as tea and wine. To fully exploit the products made from common buckwheat flower, ultra-performance liquid chromatography–electrospray ionization– tandem mass spectrometry (UPLC–ESI–MS/MS) was conducted to analyze the metabolites in red- (‘Guihong 2’) and white-flowered (‘Fengtian 1’) buckwheat cultivars. A total of 784 metabolites were identified of which flavonoids were the largest group with 191 components, followed by organic acids and derivatives (126), and amino acids and derivatives (95). Besides, dozens of phenylpropanoids, nucleotides… More >

  • Open Access


    Transcriptome analysis of purple pigment formation in Colocasia esculenta


    BIOCELL, Vol.45, No.3, pp. 785-796, 2021, DOI:10.32604/biocell.2021.014418

    Abstract Taro (Colocasia esculenta (L.) Schott) is an important crop in Africa, Southeast Asia, and subtropics and is used as a food and medicine. The purple color pigmentation is an appealing character in taro. We sampled taro corms of the cultivar ‘Lipu Taro’ at four developmental stages, including LPYS1 (without purple pigment, 50 days of development (DOD)), LPYS2 (very few purple pigments, 75 DOD), LPYS3 (moderate purple pigments, 115 DOD) and LPYS4 (high purple pigments, 205 DOD). The purpose of our study was to identify the key genes underpinning the purple pigmentation in taro based on RNA-sequencing. Through RNA-Seq, 6453 differentially… More >

  • Open Access


    Ameliorative Role of Pre-Sowing Proline Treatment in Coriandrum sativum L. Seedlings under Mercury Toxicity

    Dhriti Kapoor1, Kapani Kavani1, Amandeep Rattan2, Marco Landi3,*, Anket Sharma4,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 489-501, 2021, DOI:10.32604/phyton.2021.012359

    Abstract Heavy metal toxicity is one of the major ecosystem concerns globally in present time and is also responsible for significant threat to agronomic crops. The current work was conducted to investigate the possible ameliorative role of proline in Coriandrum sativum L. seedlings treated with mercury (Hg). The seedlings were exposed to different concentrations of Hg (0, 0.1, 0.3 and 0.5 mM) for 20 days. The effects of pre-sowing treatment with proline were studied on C. sativum seedlings in terms of pigment (chlorophylls, carotenoids and anthocyanins), malondialdehyde (MDA), antioxidant compound (glutathione, total phenolic compounds, ascorbic acid) and osmolytes (proline, glycine betaine).… More >

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