OMER ERDOGAN¹, GULEN MELIKE DEMIRBOLAT², OZGE CEVIK^1,*

BIOCELL, Vol.47, No.5, pp. 1051-1063, 2023, DOI:10.32604/biocell.2023.028129

Abstract Background: Raloxifene, a selective estrogen receptor modulator, is also known to be a lysosomotropic agent. The bioavailability of raloxifene is around 2% due to extensive hepatic transport. Exosomes are nanosized vesicles that are naturally released from cells. Method: In this study, exosomes released from HeLa cervical cancer cells were loaded with raloxifene to increase its bioavailability, and an aptamer was attached to the exosome membrane for targeting only HeLa cells. Characterization of exosomes isolated from HeLa cells was performed by transmission electron microscopy, zeta sizer, and western blotting. In addition, the cytotoxic, apoptotic, autophagic, and lysosomotropic effects of the prepared… More >

RAHELEH TORABI¹, ABBAS ALI REZVANIPOUR², HADI ESMAEILI GOUVARCHINGHALEH³, REZA RANJBAR^4,*, MOHAMMAD HEIAT^2,*

BIOCELL, Vol.46, No.12, pp. 2537-2557, 2022, DOI:10.32604/biocell.2022.021373

Abstract Ochratoxin A (OTA), one of the most dangerous mycotoxins for human health, has been subjected to numerous studies for separation and detection in minimal amounts. Aptamers as novel recognition elements have been employed to fabricate ultrasensitive biosensors for the detection of OTA and designing delicate analytical tools. This review attempted to comprehensively examine all reported aptamer-based detection and separation platforms for ochratoxin. The most relevant databases were considered to discover all specific aptamers for dealing with OTA. Aptamer-based detection and separation devices specified for OTA were searched for, analyzed, discussed, and classified based on their specifications. The optical aptasensors have… More >