TATIANA CARLA TOMIOSSO, LAURECIR GOMES, BENEDICTO DE CAMPOS VIDAL, EDSON ROSA PIMENTEL

BIOCELL, Vol.29, No.1, pp. 47-54, 2005, DOI:10.32604/biocell.2005.29.047

Abstract The composition and organization of the extracellular matrix of ostrich articular cartilage was investigated, using samples from the proximal and distal surfaces of the tarsometatarsus. For morphological analysis, sections were stained with toluidine blue and analyzed by polarized light microscopy. For biochemical analysis, extracellular matrix components were extracted with 4 M guanidinium chloride, fractionated on DEAE-Sephacel and analyzed by SDS-PAGE. Glycosaminoglycans were analyzed by electrophoresis in agarose gels. Structural analysis showed that the fibrils were arranged in different directions, especially on the distal surface. The protein and glycosaminoglycan contents of this region were higher than in the other regions. SDS-PAGE… More >

Zhongzheng Wang^1,2, Fei Tian³, Shaobai Wang³, Songtao Ai², Tsung-Yuan Tsai^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 116-117, 2019, DOI:10.32604/mcb.2019.07690

Abstract This article has no abstract. More >

P. Muddasani¹, H-J. Im¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 159-161, 2006, DOI:10.32604/mcb.2006.003.159

Abstract This article has no abstract. More >

Leo Q. Wan^1,1, Chester Miller^1,1, X. Edward Guo^2,2, Van C. Mow^1,1,3,3

Molecular & Cellular Biomechanics, Vol.1, No.1, pp. 81-100, 2004, DOI:10.3970/mcb.2004.001.081

Abstract The triphasic constitutive law [Lai, Hou and Mow (1991)] has been shown in some special 1D cases to successfully model the deformational and transport behaviors of charged-hydrated, porous-permeable, soft biological tissues, as typified by articular cartilage. Due to nonlinearities and other mathematical complexities of these equations, few problems for the deformation of such materials have ever been solved analytically. Using a perturbation procedure, we have linearized the triphasic equations with respect to a small imposed axial compressive strain, and obtained an equilibrium solution, as well as a short-time boundary layer solution for the mechano- electrochemical (MEC) fields for such a… More >

Wangping Duan^∗, Lei Wei^†, Juntao Zhang^∗, Yongzhuang Hao^∗, Chunjiang Li^∗, Hao Li^∗, Qi Li^∗, Quanyou Zhang^‡, Weiyi Chen^‡, Xiaochun Wei^∗,§

Molecular & Cellular Biomechanics, Vol.8, No.4, pp. 253-274, 2011, DOI:10.3970/mcb.2011.008.253

Abstract The cytoskeleton network is believed to play an important role in the biomechanical properties of the chondrocyte. Ours and other laboratories have demonstrated that chondrocytes exhibit a viscoelastic solid creep behavior in vitro and that viscoelastic properties decrease in osteoarthritic chondrocytes. In this study, we aimed to understand whether the alteration of viscoelastic properties is associated with changes in cytoskeleton components of ageing chondrocytes from rabbit knee articular cartilage. Three age groups were used for this study: young (2-months-old, N=23), adult (8-months-old, N=23), and old (31-months-old, N=23) rabbit groups. Cartilage structure and proteoglycan and type II collagen content were determined… More >

Rami K Korhonen^∗,†, Sang-Kuy Han^‡, Walter Herzog^‡

Molecular & Cellular Biomechanics, Vol.7, No.3, pp. 125-134, 2010, DOI:10.3970/mcb.2010.007.125

Abstract Changes in the osmotic environment cause changes in volume of isolated cells and cells in tissue explants, and the osmotic environment becomes hypotonic in cartilage diseases such as osteoarthritis (OA). However, it is not known how cells respond to a hypotonic osmotic challenge when situated in the fully intact articular cartilage.
A confocal laser scanning microscope was used to image chondrocytes of intact rabbit patellae in an isotonic (300 mOsm) and hypotonic (172 mOsm) immersion medium. Cell volumes were calculated before and 5, 15, 60, 120 and 240 minutes after the change in saline concentration. Local tissue strains and swelling… More >

Q Wang^*, YP Zheng^∗,†, HJ Niu^∗,‡

Molecular & Cellular Biomechanics, Vol.7, No.1, pp. 45-58, 2010, DOI:10.3970/mcb.2010.007.045

Abstract This study aims to obtain osmosis-induced swelling strains of normal and proteoglycan (PG) depleted articular cartilage using an ultrasound system and to investigate the changes in its mechanical properties due to the PG depletion using a layered triphasic model. The swelling strains of 20 cylindrical cartilage-bone samples collected from different bovine patellae were induced by decreasing the concentration of bath saline and monitored by the ultrasound system. The samples were subsequently digested by a trypsin solution for approximately 20 min to deplete proteoglycans, and the swelling behaviors of the digested samples were measured again. The bi-layered triphasic model proposed in… More >

Fan Song^*

Molecular & Cellular Biomechanics, Vol.7, No.1, pp. 13-24, 2010, DOI:10.3970/mcb.2010.007.013

Abstract The electrostatic interaction effects including the interaction potential, force and torque between the neighboring chondroitin sulfate glycosaminoglycan (CS-GAG) molecular chains in the bottle brush conformation of proteoglycan aggrecan are obtained as the functions of the minimum separation distance and the mutual angle between the molecular chains based on an asymptotic solution of the Poisson-Boltzmann equation that the CS-GAGs satisfy under the normal physiological conditions of articular cartilage. The present study indicates that the electrostatic interactions are not only associated intimately with the separation distance and the mutual angle, which are shown as purely exponential in separation distance and decrease with… More >

Morakot Likhitpanichkul¹, X. Edward Guo², Van C. Mow^1,3

Molecular & Cellular Biomechanics, Vol.2, No.4, pp. 191-204, 2005, DOI:10.3970/mcb.2005.002.191

Abstract Thorough analyses of the mechano-electrochemical interaction between articular cartilage matrix and the chondrocytes are crucial to understanding of the signal transduction mechanisms that modulate the cell metabolic activities and biosynthesis. Attempts have been made to model the chondrocytes embedded in the collagen-proteoglycan extracellular matrix to determine the distribution of local stress-strain field, fluid pressure and the time-dependent deformation of the cell. To date, these models still have not taken into account a remarkable characteristic of the cartilage extracellular matrix given rise from organization of the collagen fiber architecture, now known as the tension-compression nonlinearity (TCN) of the tissue, as well… More >

Taffetani M.¹, Bertarelli E.^1,2, Gottardi R.^3,4, Raiteri R.⁵, Vena P.^1,2

CMES-Computer Modeling in Engineering & Sciences, Vol.87, No.5, pp. 433-460, 2012, DOI:10.3970/cmes.2012.087.433

Abstract Dynamic nanoindentation is a novel nanomechanical testing that is being increasingly used to characterize the frequency response of viscoelastic materials and of soft hydrated biological tissues at the micrometric and nanometric length scales. This technique is able to provide more information than those obtained by simple indentation; however, its interpretation is still an open issue for complex materials such as the case of anisotropic biological tissues that generally have a high water content. This work presents a numerical model to characterize the frequency response of poro-elastic tissues subjected to harmonic indentation loading with particular regard to the effect of geometrical… More >