WENQI CHEN^1,#, XIAOYANG CHU^2,#, YANG ZENG^3,#, YOUSHENG YAN⁴, YIPENG WANG⁴, DONGLAN SUN¹, DONGLIANG ZHANG⁵, JING ZHANG^1,*, KAI YANG^4,*

BIOCELL, Vol.47, No.7, pp. 1561-1569, 2023, DOI:10.32604/biocell.2023.028851

Abstract Background: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) has a vital role in osteogenesis. However, the mechanism underlying the regulation of ROR2 in osteogenic differentiation is still poorly comprehended. A previous study by our research group showed that a novel compound heterozygous ROR2 variation accounted for the autosomal recessive Robinow syndrome (ARRS). This study attempted to explore the impact of the ROR2: c.904C>T variant specifically on the osteogenic differentiation of BMSCs. Methods: Coimmunoprecipitation (CoIP)-western blotting was carried out to identify the interaction between ROR2 and Wnt5a. Double-immunofluorescence staining was used for determining the expressions and co-localization of ROR2 and Wnt5a… More > Graphic Abstract

CHANYUAN JIN^1,4,#, ZIYAO ZHUANG^2,4,#, LINGFEI JIA^3,4,*, YUNFEI ZHENG^2,4,*

BIOCELL, Vol.46, No.7, pp. 1717-1724, 2022, DOI:10.32604/biocell.2022.018706

Abstract Osteoporosis is a frequently occurring bone remodeling disorder worldwide with one characteristic being decreasing bone mineral density and a predisposition to bone fracture, which diminishes patients’ quality of life. Several studies showed that imbalance between the osteogenesis and adipogenesis of bone marrow mesenchymal stem cells (BMSCs) took part in the development of osteoporosis. In previous study, we found MIR22HG regulated the osteogenesis of human BMSCs positively. In this study, we found that MIR22HG was decreased during the adipogenesis of human BMSCs and exerted negative effects on adipogenesis with the involvement of Wnt/β-catenin signaling pathway both in vitro and in vivo.… More >

MIN TANG^1,#, XUELING HE^1,2,#, XINGHONG YAO¹, JIRUI WEN¹, MINGYUE BAO¹, LIANG LI^1,*

BIOCELL, Vol.46, No.6, pp. 1465-1472, 2022, DOI:10.32604/biocell.2022.018967

Abstract The present study was designed to investigate the role of estrogen receptor α (ERα) in biaxial tensile strain (BTS) regulated osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs). rBMSCs were derived from rats and overexpressed ERα. The rBMSCs were subjected to BTS at 1 Hz with a strain of 2% for 4 h per day, 3 days, with or without ERα inhibitor ICI 182,780 (ICI). Then, bone mineralization was performed by Alizarin Red Staining. The markers of osteogenic differentiation and downstream Wnt3a/β-catenin signaling were detected by western blotting. Results showed that BTS enhanced the osteogenic differentiation of rBMSCs,… More >

YUKI MATSUSHITA, WANIDA ONO, NORIAKI ONO^*

BIOCELL, Vol.46, No.5, pp. 1157-1162, 2022, DOI:10.32604/biocell.2022.018960

Abstract Single-cell sequencing technologies have rapidly progressed in recent years, and been applied to characterize stem cells in a number of organs. Somatic (postnatal) stem cells are generally identified using combinations of cell surface markers and transcription factors. However, it has been challenging to define micro-heterogeneity within “stem cell” populations, each of which stands at a different level of differentiation. As stem cells become defined at a single-cell level, their differentiation path becomes clearly defined. Here, this viewpoint discusses the potential synergy of single-cell sequencing analyses with in vivo lineage-tracing approaches, with an emphasis on practical considerations in stem cell biology. More >