M. F. Elkady^1,2,*, E. M. El-Sayed², Mahmoud Samy³, Omneya A. Koriem¹, H. Shokry Hassan^4,5

Journal of Renewable Materials, Vol.12, No.2, pp. 369-380, 2024, DOI:10.32604/jrm.2023.046585

Abstract In this study, green zinc oxide (ZnO)/polypyrrole (Ppy)/cellulose acetate (CA) film has been synthesized via solvent casting. This film was used as supporting material for glucose oxidase (GOx) to sensitize a glucose biosensor. ZnO nanoparticles have been prepared via the green route using olive leaves extract as a reductant. ZnO/Ppy nanocomposite has been synthesized by a simple in-situ chemical oxidative polymerization of pyrrole (Py) monomer using ferric chloride (FeCl₃) as an oxidizing agent. The produced materials and the composite films were characterized using X-ray diffraction analysis (XRD), scanning electron microscope (SEM), Fourier transform infrared (FTIR) and thermogravimetric analysis (TGA). Glucose… More > Graphic Abstract

HAIYING CHEN, YUQING WU, BAOJIAN HUANG, LEI HAN^*

BIOCELL, Vol.47, No.6, pp. 1213-1223, 2023, DOI:10.32604/biocell.2023.028035

Abstract Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane, which can be used to redesign the cell surface with functional proteins and peptides. Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins, that are widely applied as the core elements in the field of biosensors due to their advantages, including enhanced stability, high yield, good safety, expression of larger and more complex proteins. To further promote the performance of biosensors, the biomineralized microbial surface display technology was proposed. This review summarized the… More >

Jessica E. Koehne, Jun Li¹, Alan M. Cassell, Hua Chen, Qi Ye, Jie Han, M. Meyyappan

Molecular & Cellular Biomechanics, Vol.1, No.1, pp. 69-80, 2004, DOI:10.3970/mcb.2004.001.069

Abstract Vertically aligned multi-walled carbon nanotubes (MWCNTs) have been reported in fabricating nanoelectrode arrays. Further studies on optimizing this system for the development of ultrasensitive DNA sensors are reported here. The mechanical stability of the as-grown MWCNT array can be improved by polymer coating or SiO₂ encapsulation. The latter method provides excellent electronic and ionic insulation to the sidewall of MWCNTs and the underlying metal layer, which is investigated with electrochemical impedance spectroscopy. The insulation ensures well-defined nanoelectrode behavior. A method is developed for selectively functionalizing biomolecules at the open end of MWCNTs while keeping the SiO₂ surface passivated, using the… More >