Lev A. Elkonin^1,*, Grigoriy A. Gerashchenkov²

Phyton-International Journal of Experimental Botany, Vol.95, No.8, 2026, DOI:10.32604/phyton.2026.081317 - 28 August 2026

Abstract Sorghum is a heat- and drought-tolerant cereal crop used for feed and food purposes in more than 100 countries. Sorghum becomes highly important in conditions of climate warming. The use of genome editing technologies is of paramount importance for this crop, which has a number of constraints in key agronomic traits that are difficult for improvement using conventional breeding. Application of genome editing for sorghum is limited due to problems of genetic transformation; however, recent advances in this field have increased the efficiency of the genome editing procedure in sorghum. This review summarizes recent achievements More >

Xuebing Zhu^1,2, Yongqiang Jiang^1,2, Wei Li^1,2, Lihui Wang^1,2, Xuemei Sun^1,2,*

Phyton-International Journal of Experimental Botany, Vol.95, No.7, 2026, DOI:10.32604/phyton.2026.083772 - 30 July 2026

Abstract Although transcriptional regulators of anthocyanin biosynthesis have been studied in many crops, the regulatory network controlling the purple leaf trait in lettuce (Lactuca sativa L.) remains incompletely understood. Our previous transcriptome analysis identified LsMYB1. This study further carried out the cloning and structural feature analysis of the LsMYB1 gene, and systematically verified its function using the tobacco heterologous overexpression system and CRISPR/Cas9 gene editing technology. Sequence analysis revealed a structural difference between the two alleles: the LsMYB1 allele in the purplecultivar contains two introns and three exons, whereas the allele in the greencultivar comprises only a single exon. More >

Yan Song^1,2,3,#, Yiting Chen^4,#, Xiaojing Li², Peng Pan², Ningfan Hu², Lanyi Wei², Yue Xiao^1,3,*, Chaogang Wei^2,*

BIOCELL, Vol.49, No.7, pp. 1207-1223, 2025, DOI:10.32604/biocell.2025.065658 - 25 July 2025

Abstract Objectives: Progression to castration-resistant prostate cancer (CRPC) and metastasis are the greatest challenges to effective treatment. Anticancer strategies targeting the key kinases associated with the development of CRPC may represent a breakthrough. The tyrosine kinase receptor Erythropoietin-producing hepatocellular (Eph) A2 receptor is highly expressed in CRPC cell lines and may be associated with tumor invasion and metastasis. However, the effects and exact mechanisms of EphA2 in CRPC are only partially understood. This study aimed to investigate the impact of EphA2 on CRPC cell behaviors and underlying molecular pathways Methods: CRISPR/Cas9-mediated gene editing induced EphA2-disrupted in… More >

Dao Yao^1,#, Junming Zhou^1,#, Yashuo Wang¹, Yuxin Li¹, Wenge Cheng², Xiaoyu Lu^2,*, Huijing Liu^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.6, pp. 1669-1694, 2025, DOI:10.32604/phyton.2025.064344 - 27 June 2025

Abstract Clustered regularly interspaced short palindromic repeat sequences (CRISPR) and their accompanying proteins (Cas), commonly presenting in bacteria and archaea, make up the CRISPR/Cas system. As one of the fundamental sources of nutrition for humans, edible crops play a crucial role in ensuring global food security. CRISPR/Cas9 gene editing has been applied to improve many crop traits, such as increasing nitrogen utilization efficiency, creating male sterile germplasm, and regulating tiller and spikelet formation. This paper provides a comprehensive overview of the use of CRISPR/Cas gene editing technology in crop genomes, covering the targeted genes, the types More >

LIUJIE CHEN^1,#, LILI DUAN^1,2,#, JIA LI^1,2, JUN CHEN^1,2, DUANFANG LIAO³, NONGYUE HE⁴, KAI LI^1,3, ZHENG HU^1,2,*

BIOCELL, Vol.49, No.1, pp. 21-43, 2025, DOI:10.32604/biocell.2024.056698 - 24 January 2025

Abstract Nucleic acid analysis is a key technique that enables accurate detection of various microorganisms. Conventional nucleic acid testing typically requires access to specialized laboratories, equipment, and trained personnel, which hinders the widespread use of on-site testing for DNA and RNA targets. However, integrating gene editing technology with traditional nucleic acid detection methods, especially isothermal amplification technology, can help overcome the limitations associated with on-site testing. This combination can accomplish precise and swift detection of nucleic acid sequences, offering a robust tool for on-site detection. The Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas) technology, which More >

ELHAM AMJAD¹, RAFFAELE PEZZANI^2,3,*, BABAK SOKOUTI^1,*

Oncology Research, Vol.32, No.3, pp. 439-461, 2024, DOI:10.32604/or.2023.044473 - 06 February 2024

Abstract Noncoding RNAs instruct the Cas9 nuclease to site-specifically cleave DNA in the CRISPR/Cas9 system. Despite the high incidence of hepatocellular carcinoma (HCC), the patient’s outcome is poor. As a result of the emergence of therapeutic resistance in HCC patients, clinicians have faced difficulties in treating such tumor. In addition, CRISPR/Cas9 screens were used to identify genes that improve the clinical response of HCC patients. It is the objective of this article to summarize the current understanding of the use of the CRISPR/Cas9 system for the treatment of cancer, with a particular emphasis on HCC as… More >

Hafiza Arooj Razzaq¹, Siddra Ijaz^1,*, Imran Ul Haq², Faisal Saeed Awan¹

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2451-2460, 2023, DOI:10.32604/phyton.2023.030501 - 25 June 2023

Abstract The imperative aspect of the CRISPR/Cas9 system is a short stretch of 20 nucleotides of gRNA that control the overall specificity. Due to the small size, the chance of its multiple occurrences in the genome increases; however, a few mismatches are tolerated by the Cas9 endonuclease activity. An accurate and careful in silico-based off-target prediction while target selection is preferred to address the issue. These predictions are based on a comprehensive set of selectable parameters. Therefore, we investigated the possible off-target prediction and their screening in StERF3 gene-edited potato plants while developing StERF3-loss-of-function mutants using CRISPR/Cas9 More >

Shuo Qu¹, Qi Cai², Huimin Cui¹, Lamboro Abraham¹, Yaolei Jiao¹, Guilong Ma², Piwu Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 501-519, 2023, DOI:10.32604/phyton.2022.023479 - 12 October 2022

Abstract High yield, high quality, stable yield, adaptability to growth period, and modern mechanization are the basic requirements for crops in the 21st century. Soybean oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and stability. Known as a safe fatty acid, it has the ability to successfully prevent cardiovascular and cerebrovascular disorders. Improving the fatty acid composition of soybean seeds, can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans, but also have important significance in human health, and provide the possibility for the development of soybean oil… More >

MINGZHAO LI¹, QIAN ZHANG², WENBIN HUANG¹, SHIYING ZHANG¹, NAN JIANG², XIAOSHUAI HUANG^3,*, FENG CHEN^2,*

BIOCELL, Vol.47, No.1, pp. 205-214, 2023, DOI:10.32604/biocell.2022.022572 - 26 September 2022

Abstract Cancer progression involves the sonic hedgehog (SHH) pathway, in which the receptor PTCH1 actives the downstream pathways. Dysfunction of PTCH1 can lead to nevoid basal cell carcinoma Syndrome (NBCCs) including neoplastic disease and congenital disorder. To evaluate the relationship between PTCH1 and cancer, we applied the CRISPR/Cas9 system to knock out PTCH1 in oral nontumorous epithelial cells (GMSM-K). Then we screened six PTCH1 variants associated with cleft lip/palate (CL/P), one of the congenital disorders in NBCCs, and generated PTCH1 variant and wild-type recombinant PTCH1^−/− GMSM-K cell lines. Transcriptome sequencing was conducted in these cell lines. The… More >

RUYU YAN^1,#, JUNJIE WANG^1,#, MINXIA LIU², KECHENG ZHOU^1,3,*

BIOCELL, Vol.46, No.10, pp. 2159-2165, 2022, DOI:10.32604/biocell.2022.021107 - 13 June 2022

Abstract Emerging cohorts and basic studies have associated certain genetic modifications in cancer patients, such as gene mutation, amplification, or deletion, with the overall survival prognosis, underscoring patients’ genetic background may directly regulate drug sensitivity/resistance during chemotherapies. Understanding the molecular mechanism underpinning drug sensitivity/resistance and further uncovering the effective drugs have been the major ambition in the cancer drug discovery. The emergence and popularity of CRISPR/Cas9 technology have reformed the entire life science research, providing a precise and simplified genome editing tool with unlimited editing possibilities. Furthermore, it presents a powerful tool in cancer drug discovery, More >