Open Access iconOpen Access

REVIEW

Advances in CRISPR-based gene editing technology and its application in nucleic acid detection

by LIUJIE CHEN1,#, LILI DUAN1,2,#, JIA LI1,2, JUN CHEN1,2, DUANFANG LIAO3, NONGYUE HE4, KAI LI1,3, ZHENG HU1,2,*

1 Translational Medicine Institute, the First People’s Hospital of Chenzhou, Hengyang Medical School, University of South China, Chenzhou, 423000, China
2 The First Clinical College of Xiangnan University, The First Affiliated Hospital of Xiangnan University, Chenzhou, 423000, China
3 National Engineering Research Center of Personalized Diagnostic and Therapeutic Technology, Hunan University of Chinese Medicine, Changsha, 410208, China
4 State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China

* Corresponding Author: ZHENG HU. Email: email
# These authors contributed equally to this work

BIOCELL 2025, 49(1), 21-43. https://doi.org/10.32604/biocell.2024.056698

Abstract

Nucleic acid analysis is a key technique that enables accurate detection of various microorganisms. Conventional nucleic acid testing typically requires access to specialized laboratories, equipment, and trained personnel, which hinders the widespread use of on-site testing for DNA and RNA targets. However, integrating gene editing technology with traditional nucleic acid detection methods, especially isothermal amplification technology, can help overcome the limitations associated with on-site testing. This combination can accomplish precise and swift detection of nucleic acid sequences, offering a robust tool for on-site detection. The Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas) technology, which comprises the CRISPR system and Cas effector proteins, is a powerful tool that is advancing the field of nucleic acid detection. Specifically, Cas12, Cas13, and Cas14 proteins have emerged as straightforward, effective, precise, sensitive, and cost-effective methods for in vitro nucleic acid detection because of their “collateral cleavage” characteristics. When combined with the “collateral cleavage” ability of Cas protein and isothermal amplification, CRISPR/Cas systems have great potential to advance nucleic acid detection. This article summarizes the research progress of different CRISPR/Cas systems and their applications in nucleic acid detection and future perspectives.

Keywords


Cite This Article

APA Style
CHEN, L., DUAN, L., LI, J., CHEN, J., LIAO, D. et al. (2025). Advances in crispr-based gene editing technology and its application in nucleic acid detection. BIOCELL, 49(1), 21–43. https://doi.org/10.32604/biocell.2024.056698
Vancouver Style
CHEN L, DUAN L, LI J, CHEN J, LIAO D, HE N, et al. Advances in crispr-based gene editing technology and its application in nucleic acid detection. BIOCELL. 2025;49(1):21–43. https://doi.org/10.32604/biocell.2024.056698
IEEE Style
L. CHEN et al., “Advances in CRISPR-based gene editing technology and its application in nucleic acid detection,” BIOCELL, vol. 49, no. 1, pp. 21–43, 2025. https://doi.org/10.32604/biocell.2024.056698



cc Copyright © 2025 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 1792

    View

  • 589

    Download

  • 0

    Like

Share Link