MINGZHAO LI1, QIAN ZHANG2, WENBIN HUANG1, SHIYING ZHANG1, NAN JIANG2, XIAOSHUAI HUANG3,*, FENG CHEN2,*
BIOCELL, Vol.47, No.1, pp. 205-214, 2023, DOI:10.32604/biocell.2022.022572
Abstract Cancer progression involves the sonic hedgehog (SHH) pathway, in which the receptor PTCH1 actives the
downstream pathways. Dysfunction of PTCH1 can lead to nevoid basal cell carcinoma Syndrome (NBCCs) including
neoplastic disease and congenital disorder. To evaluate the relationship between PTCH1 and cancer, we applied the
CRISPR/Cas9 system to knock out PTCH1 in oral nontumorous epithelial cells (GMSM-K). Then we screened six
PTCH1 variants associated with cleft lip/palate (CL/P), one of the congenital disorders in NBCCs, and generated
PTCH1 variant and wild-type recombinant PTCH1−/− GMSM-K cell lines. Transcriptome sequencing was conducted
in these cell lines. The results revealed that differentially… More >
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