Maria Khizar1, Urooj Haroon1, Musrat Ali1, Samiah Arif2, Iftikhar Hussain Shah2 , Hassan Javed Chaudhary1, Muhammad Farooq Hussain Munis1,*
Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 103-109, 2020, DOI:10.32604/phyton.2020.08010
Abstract Cotton (Gossypium hirsutum L.) is a key fiber crop of great commercial
importance. Numerous phytopathogens decimate crop production by causing
various diseases. During July-August 2018, leaf spot symptoms were recurrently
observed on cotton leaves in Rahim Yar Khan, Pakistan and adjacent areas. Infected
leaf samples were collected and plated on potato dextrose agar (PDA) media.
Causal agent of cotton leaf spot was isolated, characterized and identified as
Aspergillus tubingensis based on morphological and microscopic observations.
Conclusive identification of pathogen was done on the comparative molecular
analysis of CaM and β-tubulin gene sequences. BLAST analysis of both sequenced
genes showed 99%… More >
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