JIANRONG YANG1,#, WAN CHEN2,#, XING ZHOU3,#, YAOXUAN LI4,#, ZHIHUANG NONG5, LIYUAN ZHOU4, XUAN WEI4, XIAORONG PAN6, CHUNXIA CHEN7, WENSHENG LU3
BIOCELL, Vol.46, No.1, pp. 137-148, 2022, DOI:10.32604/biocell.2022.016807
Abstract In this study, we investigated the protective effect of hyperbaric oxygen (HBO) on PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion and its possible mechanism. PC12 and H9C2 cell oxygen-glucose deprivation/reperfusion model were established. Cells were divided into a control group, model group, hyperbaric air (HBA) group and HBO group. The cell viability was detected by the CCK8 assay. Hoechst 33342 and PI staining assays and mitochondrial membrane potential (MMP) assays were used to detect cell apoptosis. The ultrastructure of cells, including autophagosomes, lysosomes, and apoptosis, were examined using a transmission electron microscope. The expression of autophagy-related proteins was… More >
Login
Register
