KARINA M. MICHETTI1,2, PATRICIA I. LEONARDI1,3, EDUARDO J. CÁCERES1,4
BIOCELL, Vol.30, No.3, pp. 491-496, 2006, DOI:10.32604/biocell.2006.30.491
Abstract Nonspecific acid phosphatases are a group of enzymes whose activity increases the availability of exogenous and endogenous orthophosphate either through extra- or intracellular hydrolysis of phosphate compounds. Our study demonstrates the activity of acid phosphatases in the filamentous freshwater alga Stigeoclonium tenue. These enzymes were detected following a cerium-based method in which cerium was used as an orthophosphate-capture reagent. In thalli from S. tenue from the natural environment, acid phosphatases were found in the longitudinal cell wall, plasmalemma, and vacuole. In thalli from Bold’s Basal Medium culture, these enzymes were found mainly in the plasmalemma; they were scarce in the… More >
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