SAYAKA IMATSUJI^1,#, YUKIKO UJIE^1,#, HIROYUKI ODAKE¹, MASAYA IMOTO^1,2, SUSUMU ITOH³, ETSU TASHIRO^1,3,*

Oncology Research, Vol.32, No.1, pp. 139-150, 2024, DOI:10.32604/or.2023.030190

Abstract Growing evidence suggests an association between epithelial-mesenchymal transition (EMT), a hallmark of tumor malignancy, and chemoresistance to a number of anti-cancer drugs. However, the mechanism of EMT induction in the process of acquiring anti-cancer drug resistance remains unclear. To address this issue, we obtained a number of cisplatin-resistant clones from LoVo cells and found that almost all of them lost cell-cell contacts. In these clones, the epithelial marker E-cadherin was downregulated, whereas the mesenchymal marker N-cadherin was upregulated. Moreover, the expression of EMT-related transcription factors, including Slug, was elevated. On the other hand, the upregulation of other mesenchymal marker Vimentin… More >

LIANLI NI^1,2,#, YUN YU^1,2,#, HAN LIN^1,2, WEISHAN ZHUGE², LU TAO², YIWEI SHEN², RI CUI^2,*, SHAOTANG LI^1,2,*

BIOCELL, Vol.47, No.10, pp. 2245-2254, 2023, DOI:10.32604/biocell.2023.030147

Abstract Background: Homeobox B8 (HOXB8), a member of HOX family, plays a key role in the development of colorectal cancer (CRC). However, the function of HOXB8 in oxaliplatin (OXA) resistance in CRC is still unclear. This study investigated the role and precise molecular mechanism of HOXB8 in OXA-resistant CRC cells. Methods: The cell viability was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, and the colony forming ability was determined by colony formation assay. The silencing RNA (siRNA) approach was used to knockdown HOXB8 in CRC cells while the lentiviral transfection system was used to establish stable HOXB8 overexpressing CRC… More >