Adel A. Rezk^1,2,*, Hala A. Amin²

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 691-705, 2023, DOI:10.32604/phyton.2022.024208

Abstract Citrus Tristeza Virus (CTV), usually occurs in nature as a mixture of genotypes. Six naturally infected citrus (Citrus sinensis) trees grafted on sour orange rootstock were collected from three citrus growing governorates in Egypt (Sharqia, Qalyubia and Garbia). In this study, RT-PCR, Single-Strand Conformation Polymorphism (SSCP) and nucleotide sequence analysis were used for four independent CTV genomic regions (p65, p18, p20, and p23) to detect and assess the sequence and genetic variabilities among CTV Egyptian isolates. RTPCR products (650 bp) for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis and DNA sequencing. SSCP… More >

Yasir Iftikhar^1,*, Mazhar Abbas², Mustansar Mubeen³, Muhammad Zafar-ul-Hye^4,*, Faheema Bakhtawar¹, Sonum Bashir¹, Ashara Sajid¹, Muhammad Asif Shabbir¹

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1063-1074, 2021, DOI:10.32604/phyton.2021.015508

Abstract Tristeza is a devastating viral disease in all the citrus growing countries throughout the world and has killed millions of citrus trees in severely affected orchards. The citrus species grafted on sour orange rootstock are affected by this disease. Predominantly, the sweet orange, grapefruit and lime trees grafted on sour orange exhibit severe symptoms like quick decline, vein clearing, pin holing, bark scaling and degeneration leading to variable symptoms. Symptomatic expression of Citrus tristeza virus (CTV) in different hosts has been attributed to virus isolates which are from severe to mild. Different serological and molecular assays have been deployed to… More >