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    ARTICLE

    Development of polymorphic SSR markers and their applicability in genetic diversity evaluation in Euptelea pleiosperma

    XIAOJUN ZHOU*, XIAOYU LU, XUBO WANG

    BIOCELL, Vol.46, No.11, pp. 2489-2495, 2022, DOI:10.32604/biocell.2022.020311

    Abstract Euptelea pleiosperma is a characteristic species of East Asian flora with both ornamental and scientific values. Based on the reduced-representation sequencing (RRS) technology of RAD-Seq, this study conducted high-throughput Illumina paired-end sequencing to find SSR marker information in the genome of E. pleiosperma, and to screen and verify polymorphism of SSR markers. We obtained 5.5G of high-quality data using RAD-Seq. The total number of contigs of the RAD tags was 299,376, with the maximum contig length of 2,062 bp and the average length of 445 bp. From these sequences, we identified 20,718 SSR loci, with a distribution density of one… More >

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