WENJUAN XU1, GUANGMING DAI1, YIFEI LYU2, YINING ZHANG1, XIAOLIN TU1,*
BIOCELL, Vol.46, No.4, pp. 1097-1104, 2022, DOI:10.32604/biocell.2022.017505
Abstract This study established a method for isolating large numbers of high-purity osteocytes from high-density bone. Bone fragments derived from mice tibia and femurs were alternately digested with type I collagenase and EDTA nine times, and the digested cells and bone chips (BC) were cultured, digested, and passaged when cells were fully grown. The types of cells obtained were identified by morphology, viable cell counts, alkaline phosphatase staining, and biochemical activity analyses, and specific osteocyte and osteoblast markers were evaluated by quantitative real-time polymerase chain reaction. Our results showed that among the cells obtained from the third digestion (fractions 7–9) of… More >
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