Farshid GOLSHANI1, Barat Ali FAKHERI1, Mahmood SOLOUKI1, Nafiseh MAHDINEZHAD1, Majid Reza KIANI FERIZ2
BIOCELL, Vol.43, No.3, pp. 225-232, 2019, DOI:10.32604/biocell.2019.07547
Abstract ITS (Internal Transcription Spacer of nuclear ribosomal DNA) regions method was applied to identify and
study the phylogenetic relationships existing between 41 individuals of Crocus, including 8 species collected in Iran (4
crop and 8 wild individuals) and 16 species (2 crop and 27 wild individuals) already registered in GenBank (NCBI).
PCR amplifications were performed using the primer designed on the nucleotide sequence of nuclear region (ITS).
A total of 12 sequences were obtained and registered in NCBI database. In particular, 4 of these sequences were not
already present in the scientific library. Nucleotide polymorphic sites were counted for ITS… More >
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