Jianquan Ouyang1,*, Jinling Wang1, Yaowu Wang1, Tianming Liu2
Journal of Cyber Security, Vol.4, No.2, pp. 65-77, 2022, DOI:10.32604/jcs.2022.028065
Abstract Cryo-electron microscopy (cryo-EM) has become one of the mainstream techniques for determining the structures of proteins and macromolecular complexes, with prospects for development and significance. Researchers must select hundreds of thousands of particles from micrographs to acquire the database for single-particle cryo-EM reconstruction. However, existing particle picking methods cannot ensure that the particles are in the center of the bounding box because the signal-to-noise ratio (SNR) of micrographs is extremely low, thereby directly affecting the efficiency and accuracy of 3D reconstruction. We propose an automated particle-picking method (CenterPicker) based on particle center point detection to automatically select a large number… More >
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