Xinyue Wang1,#, Jing Xu1,#, Qihong Gu1, Dingxuan Tang1, Huoyan Ji2, Shaoqing Ju2, Feng Wang2,*, Lin Chen3, Ruoyu Yuan2,*
Oncologie, Vol.24, No.3, pp. 513-526, 2022, DOI:10.32604/oncologie.2022.024373
Abstract Background: MicroRNAs (miRNAs) have been identified as promising novel biomarkers for cancer diagnosis and
prognosis, especially for hepatocellular carcinoma (HCC). Nowadays, the expression level of miR-21 in serum
samples is a diagnostic indicator for HCC diagnosis. Thus, the quantitative determination of miRNA concentration is of significance in clinical practice. It is particularly important to establish an analytical detection method
for miR-21 in patient serum. Methods: The signal readout for miR-21 was based on the mass response of a reporter peptide using an isotope dilution mass spectrometry (MS) method in this work. To be more specific, miR-21
was biotinylated before being… More >
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