WEI LU1,*, KAN GUO2, DIANMEI XI1, ZHAOXIA XIA1
BIOCELL, Vol.47, No.9, pp. 1995-2008, 2023, DOI:10.32604/biocell.2023.024591
Abstract Background: Mesenchymal stem cell (MSC)-derived exosomes are closely related to pyroptosis in diabetic
nephropathy (DN). This study aimed to explore the protective effect of exosomal miR-30a-5p on podocyte pyroptosis
in DN. Methods: Streptozotocin was used to establish the mouse model of DN. Human bone marrow MSC-derived
exosomes were extracted and identified via transmission electron microscopy, nanoparticle tracking analysis, and
western blotting. MiR-30a-5p mimics and non-control (NC) mimics were transfected into MSCs and podocytes, and
exosomes were isolated from the MSCs. High glucose (HG)-induced podocyte model was established to determine the
effect of exosomal miR-30a-5p on pyroptosis and inflammation in vitro.… More >
Login
Register
