Jing Cai^1,#, Haoyun Zhu^1,#, Weiling Guo¹, Ting Huang¹, Pangzhou Chen^1,2, Wen Zhou¹, Ziyun Guan^1,3,*

Oncology Research, Vol.34, No.1, 2026, DOI:10.32604/or.2025.069902 - 30 December 2025

Abstract Background: Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity. Current preclinical models, however, are inadequate for predicting individual patient responses towards different drugs. This study aimed to investigate the patient-derived breast cancer culture models for drug sensitivity evaluations. Methods: Tumor and adjacent tissues from female breast cancer patients were collected during surgery. Patient-derived breast cancer cells were cultured using the conditional reprogramming technique to establish 2D models. The obtained patient-derived conditional reprogramming breast cancer (CRBC) cells were subsequently embedded in alginate-gelatin methacryloyl hydrogel microspheres… More >

Shuyan Kong^1,#, Danting Li^1,#, Haonan Meng^1,#, Haoxiang Zhang¹, Xiaochun Jiang¹, Peilin Zheng², Shoujun Huang^1,*

BIOCELL, Vol.49, No.8, pp. 1481-1504, 2025, DOI:10.32604/biocell.2025.066205 - 29 August 2025

Abstract Background: Small ubiquitin-like modifier (SUMO)-specific proteases (SENPs) cleave the isopeptidic bond between SUMO1/2/3 and protein substrates, thus regulating the structure, activity, and lifetime of a variety of proteins. Recently, accumulating evidence has suggested that SENPs play a role in the initiation and progression of human cancers. Nevertheless, the potential role of the SENP family of proteins in liver cancer has yet to be fully elucidated. Methods: This study conducted a comprehensive bioinformatics analysis of the SENP family in liver cancer, including differential expression profiling, survival analysis, mutation and copy number variations (CNVs) assessment, immune infiltration… More >

Zhun Yu^1,2, Jie Wang^1,2, Guoping Xu^1,2,*

Oncology Research, Vol.33, No.8, pp. 2013-2035, 2025, DOI:10.32604/or.2025.061757 - 18 July 2025

Abstract Objectives: Triple-negative breast cancer (TNBC) presents a major treatment challenge due to its aggressive behavior. The dysfunction of the Golgi apparatus (GA) contributes to the development of various cancers. This study aimed to utilize GA-related genes (GARGs) to forecast the prognosis and immune profile of TNBC. Methods: The data were downloaded from The Cancer Genome Atlas (TCGA) database, including 175 TNBC and 99 healthy samples. The differentially expressed GARGs (DEGARGs) were analyzed using the TCGA biolinks package. The patients with TNBC were classified into two clusters utilizing the ConsensusClusterPlus package according to prognosis-related DEGARGs, followed by… More >

HONGYUAN LIANG^1,#, YANQIU LI^2,#, YONGGANG QU³, LINGYUN ZHANG^4,*

Oncology Research, Vol.32, No.2, pp. 393-407, 2024, DOI:10.32604/or.2023.031134 - 28 December 2023

Abstract Advanced LUAD shows limited response to treatment including immune therapy. With the development of sequencing omics, it is urgent to combine high-throughput multi-omics data to identify new immune checkpoint therapeutic response markers. Using GSE72094 (n = 386) and GSE31210 (n = 226) gene expression profile data in the GEO database, we identified genes associated with lung adenocarcinoma (LUAD) death using tools such as “edgeR” and “maftools” and visualized the characteristics of these genes using the “circlize” R package. We constructed a prognostic model based on death-related genes and optimized the model using LASSO-Cox regression methods.… More >

QIOU GU, TIAN ZHAN, XIAO GUAN, CHUILIN LAI, NA LU, GUOGUANG WANG, LEI XU, XIANG GAO, JIANPING ZHANG^*

Oncology Research, Vol.31, No.3, pp. 287-297, 2023, DOI:10.32604/or.2023.028124 - 22 May 2023

Abstract Background: Gastric cancer (GC) is a malignancy with the worst prognosis that seriously threatens human health, especially in East Asia. Apolipoprotein C1 (apoc1) belongs to the apolipoprotein family. In addition, apoc1 has been associated with various tumors. However, its role in GC remains unclear. Methods: Firstly, we quantified its expression in GC and adjacent tumor tissues, using The Cancer Genome Atlas (TCGA). Next, we assessed cell invasion and migration abilities. Finally, we revealed the role of apoc1 in the tumor microenvironment (TME), immune cell infiltration and drug sensitivity. Results: Firstly, in TCGA database, it has been shown that… More >

RUYU YAN^1,#, JUNJIE WANG^1,#, MINXIA LIU², KECHENG ZHOU^1,3,*

BIOCELL, Vol.46, No.10, pp. 2159-2165, 2022, DOI:10.32604/biocell.2022.021107 - 13 June 2022

Abstract Emerging cohorts and basic studies have associated certain genetic modifications in cancer patients, such as gene mutation, amplification, or deletion, with the overall survival prognosis, underscoring patients’ genetic background may directly regulate drug sensitivity/resistance during chemotherapies. Understanding the molecular mechanism underpinning drug sensitivity/resistance and further uncovering the effective drugs have been the major ambition in the cancer drug discovery. The emergence and popularity of CRISPR/Cas9 technology have reformed the entire life science research, providing a precise and simplified genome editing tool with unlimited editing possibilities. Furthermore, it presents a powerful tool in cancer drug discovery, More >

Sheng Li, Zhengli Cui, Xianfeng Meng

Oncology Research, Vol.24, No.4, pp. 279-286, 2016, DOI:10.3727/096504016X14666990347554

Abstract Poly(ADP-ribose) polymerase 1 (PARP-1) is reported to be involved in DNA repair and is now recognized as a key regulator in carcinogenesis. However, the potential role and the molecular mechanism underlying the effect of PARP-1 on osteosarcoma (OS) cells have not been elucidated. In this study, the results showed that knockdown of PARP-1 resulted in decreased cell proliferation, increased cell apoptosis, and G₀/G₁ phase arrest in U2OS cells. In addition, increased expression of active caspase 3 and Bax, but reduced Bcl-2, cyclin D1, and phosphorylated extracellular signal regulated kinase 1/2 (pERK1/2) were observed in PARP-1 knockdown More >

Qun Zhao, Bi-Bo Tan, Yong Li, Li-Qiao Fan, Pei-Gang Yang, Yuan Tian

Oncology Research, Vol.23, No.3, pp. 129-136, 2015, DOI:10.3727/096504015X14500513118029

Abstract In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OCUM-2MD3 and gastric epithelial cells. After HIF-1α-siRNA More >