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  • Open Access

    ARTICLE

    Brassinosteroid Biosynthetic Gene CmDWF4 Regulates Bud Outgrowth in Chrysanthemum morifolium

    Xianrong Fu, Aiping Song, Bo Peng, Song Li, Weixin Liu, Lingling Zhang, Jiafu Jiang, Sumei Chen, Fadi Chen*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1681-1694, 2023, DOI:10.32604/phyton.2023.027870

    Abstract Brassinosteroids (BRs), a class of steroid phytohormones, play a critical role in plant growth and development. The DWF4 gene encodes a cytochrome P450 enzyme (CYP90B1), which is considered a rate-limiting enzyme in BR biosynthesis. Here, we identified a homologous gene of DWF4 in chrysanthemum, CmDWF4. This gene was predicted to encode 491 amino acid residues with a molecular weight of 56.2 kDa and an isoelectric point (pI) of 9.10. Overexpression of CmDWF4 in chrysanthemum was found to significantly increase growth rate, number, and length of lateral buds. Transcriptome analysis showed that multiple xyloglucan endotransglycosylase/hydrolase (XTH) family encoding genes associated with… More >

  • Open Access

    ARTICLE

    Adaptive Emulation Framework for Multi-Architecture IoT Firmware Testing

    Jihyeon Yu1, Juhwan Kim1, Youngwoo Lee1, Fayozbek Rustamov2, Joobeom Yun1,*

    CMC-Computers, Materials & Continua, Vol.75, No.2, pp. 3291-3315, 2023, DOI:10.32604/cmc.2023.035835

    Abstract Internet of things (IoT) devices are being increasingly used in numerous areas. However, the low priority on security and various IoT types have made these devices vulnerable to attacks. To prevent this, recent studies have analyzed firmware in an emulation environment that does not require actual devices and is efficient for repeated experiments. However, these studies focused only on major firmware architectures and rarely considered exotic firmware. In addition, because of the diversity of firmware, the emulation success rate is not high in terms of large-scale analyses. In this study, we propose the adaptive emulation framework for multi-architecture (AEMA). In… More >

  • Open Access

    ARTICLE

    Comparative Non Participating Transcriptome Analysis Response to Low Phosphorus by CmPht1;2 in Chrysanthemum

    Chen Liu1,2 , Xiaowei Tang1,2, Xiaohui Qu1,2 , Fadi Chen1, Jiafu Jiang1 and Sumei Chen1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1391-1404, 2023, DOI:10.32604/phyton.2023.027231

    Abstract Chrysanthemum morifolium Ramat is one of the four major cut flowers in the world. Pht1 family is focus on the uptake and transport of phosphate in plants. In our previous studies, CmPht1;2 overexpression line (Oe1) had higher phosphate contents both in roots and shoots, and its root development was significantly enhanced than wild type (WT) at low phosphorus conditions in chrysanthemum. Metabolomics analysis showed that several metabolites had a change in pyruvate metabolism and tricarboxylic acid (TCA) cycle pathway. To explore the gene difference expression and the change of metabolic pathway between CmPht1;2-Oe1 and WT, we conducted the transcriptome analysis.… More >

  • Open Access

    ARTICLE

    Interaction of Foam and Microemulsion Components in Low-Tension-Gas Flooding

    Jing Zhao, Jun Yang*

    FDMP-Fluid Dynamics & Materials Processing, Vol.19, No.7, pp. 1951-1961, 2023, DOI:10.32604/fdmp.2023.026115

    Abstract Low-Tension-Foam (LTF) flooding is an emerging enhanced oil recovery technique for low-permeability carbonate reservoirs. Foam capacity is closely related to the salinity environment (or, equivalently, the phase behavior of the oil/water/surfactant system). Therefore, the interactions between microemulsion and foam components are of primary importance in the LTF process. In this study, the phase behavior of an oil/water/surfactant system under equilibrium is analyzed, firstly by assuming perfect mixing. Meanwhile, the formation kinetics of microemulsion are monitored through a novel low-field NMR technique, which is able to provide quantitative assessment on the microemulsion evolution characteristics. Then, foam stability is examined in the… More >

  • Open Access

    ARTICLE

    B Class Floral Homeotic Genes are Involved in the Petal Identity and Flower Meristem Determinations in Chrysanthemum morifolium

    Jiayou Liu, Lian Ding, Xue Zhang, Song Li, Yunxiao Guan, Diwen Jia, Aiping Song, Jiafu Jiang, Fadi Chen*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 311-331, 2023, DOI:10.32604/phyton.2022.023896

    Abstract Chrysanthemum morifolium, an ornamental crop with diverse forms of inflorescence, is a good model for studying flower development in Asteraceae. However, the genetic background is complex and the mechanisms of regulating flower development are still unclear. Here, we identified two natural mutant lines of chrysanthemum and named them M1 and M2 according to the severity of the phenotype. Both lines showed defects in petal identity, and the petals of the M1 line had a mild phenotype: partially loss of petal identity and conversion of petals into green, leaf-like organs. The M2 line had severe phenotypes: in addition to severe petal… More >

  • Open Access

    ARTICLE

    Enhanced Primary User Emulation Attack Inference in Cognitive Radio Networks Using Machine Learning Algorithm

    N. Sureka*, K. Gunaseelan

    Intelligent Automation & Soft Computing, Vol.34, No.3, pp. 1893-1906, 2022, DOI:10.32604/iasc.2022.026098

    Abstract Cognitive Radio (CR) is a competent technique devised to smart sense its surroundings and address the spectrum scarcity issues in wireless communication networks. The Primary User Emulation Attack (PUEA) is one of the most serious security threats affecting the performance of CR networks. In this paper, machine learning (ML) principles have been applied to detect PUEA with superior decision-making ability. To distinguish the attacking nodes, Reinforced Learning (RL) and Extreme Machine Learning (EML-RL) algorithms are proposed to be based on Reinforced Learning (EML). Various dynamic parameters like estimation error, attack detection efficiency, attack estimation rate, and learning rate have been… More >

  • Open Access

    ARTICLE

    Time-Variant Reliability-Based Multi-Objective Fuzzy Design Optimization for Anti-Roll Torsion Bar of EMU

    Pengpeng Zhi1,2,3, Zhonglai Wang1,2,*, Bingzhi Chen3, Ziqiang Sheng4

    CMES-Computer Modeling in Engineering & Sciences, Vol.131, No.2, pp. 1001-1022, 2022, DOI:10.32604/cmes.2022.019835

    Abstract Although various types of anti-roll torsion bars have been developed to inhibit excessive roll angle of the electric multiple unit (EMU) car body, it is critical to ensure the reliability of structural design due to the complexity of the problems involving time and uncertainties. To address this issue, a multi-objective fuzzy design optimization model is constructed considering time-variant stiffness and strength reliability constraints for the anti-roll torsion bar. A hybrid optimization strategy combining the design of experiment (DoE) sampling and non-linear programming by quadratic lagrangian (NLPQL) is presented to deal with the design optimization model. To characterize the effect of… More >

  • Open Access

    ARTICLE

    Genotype Screening of Recipient Resources with High Regeneration and Transformation Efficiency in Chrysanthemum

    Yajun Li1,#, Yumeng Cui1,#, Bingjie Wang1, Yue Li1, Mengmeng Zhang2, Silan Dai1, He Huang1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 869-888, 2022, DOI:10.32604/phyton.2022.018659

    Abstract Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum, which relies on an optimal regeneration and transformation system. However, the regeneration system of different chrysanthemum cultivars varies, and the regeneration time of most cultivars is long. To screen cultivars with highly efficient regeneration, leaves and shoot tip thin cell layers (tTCL) from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media (MS) supplemented with 1.0–5.0 mg L−1 6-benzylaminopurine (6-BA) and 0.1–1.0 mg L−1 α-naphthaleneacetic (NAA). The results showed that the most efficient regeneration media were MS +… More >

  • Open Access

    ARTICLE

    Numerical Simulation of Bone Plate with Fatigue Crack and Investigation of Attraction Hole for Retarding Crack Growth

    Zhonghang Zhao, Aimin Ji*, Changsheng Chen

    Molecular & Cellular Biomechanics, Vol.18, No.4, pp. 173-185, 2021, DOI:10.32604/mcb.2021.016238

    Abstract Premature fracture of the bone plate caused by fatigue crack is the main failure mode in treating femoral shaft fracture. In order to improve the durability of the plate, this study proposed a crack attraction hole (CAH) to retard the crack propagation based on the fracture mechanics. In this paper, a numerical model of the femoral fracture internal fixation system was constructed, in which the femur was developed using a validated simplified model. First, the fatigue crack initiation location was defined at the stress concentration through static analysis. Next, with the joint simulation method of Franc3D and ABAQUS, the fatigue… More >

  • Open Access

    ARTICLE

    Proteomic Analysis of Chrysanthemum Lateral Buds after Removing Apical Dominance Based on Label-Free Technology

    Sicong Zheng#, Jingjing Song#, Cheng Luo, Xin Li, Qiqi Ma, Beibei Jiang*, Qinglin Liu, Yuanzhi Pan

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 525-539, 2022, DOI:10.32604/phyton.2022.017629

    Abstract Studying the genetic basis and regulatory mechanism of chrysanthemum lateral bud outgrowth is of great significance for reduction the production cost of cut chrysanthemum. To clarify the molecular basis of lateral bud elongation after removal of apical dominance in chrysanthemum, label-free quantification analysis was used to analyze the proteome changes after apical bud removal. Quantitative real-time PCR (qPCR) was used to analyze the changes in the expression of three plant hormone-related genes. A total of 440 differentially expressed proteins were successfully identified at three time points during the lateral bud elongation. The number of differentially expressed proteins in the three… More >

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