TINGTING HUANG^1,#, SHAN DENG^2,#, JIANGYUAN SHENG¹, DI ZHANG^1,*

BIOCELL, Vol.46, No.11, pp. 2507-2515, 2022, DOI:10.32604/biocell.2022.021314

Abstract Dehydrins (DHNs), as members of the late embryogenesis abundant protein family, play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses. Vitrification is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic storage. In this study, ApSK₃ type DHN was genetically transformed into embryogenic calluses (EC) of Agapanthus praecox by overexpression (OE) and RNA interference (RNAi) techniques to evaluate the in vivo protective effect of DHNs during cryopreservation. The cell viability showed a completely opposite trend in OE and RNAi… More >

Yingbo Li^1,2,#, Ting He^1,2,#, Guimei Guo^1,2, Hongwei Xu^1,2, Yingjie Zong^1,2, Shuwei Zhang^1,2, Ruiju Lu^1,2, Longhua Zhou^1,2,*, Chenghong Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1153-1164, 2022, DOI:10.32604/phyton.2022.019520

Abstract Agrobacterium-mediated transformation has been widely used in plants. However, the mechanism in plant cells’ response to Agrobacterium infection was very complex. The mechanism of the determinants in host cell remains obscure, especially in barley, which is recalcitrant for Agrobacterium-mediated transformation. In the present study, microspore-derived embryogenic calli (MDEC) from barley elite cultivar were employed as unique subjects to characterize the mechanisms during the Agrobacterium infection process. Hua 30 MDEC can be successfully infected by Agrobacterium. RNA-sequencing at different infection points (0, 2, 6, 12, 24 hpi) was performed. The average expressional intensity of the whole genomics increased from 0 to… More >

Paul Chege^1,2, Andrea Palágyi², Csaba Lantos², Erzsébet Kiss¹, János Pauk^2,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 111-119, 2020, DOI:10.32604/phyton.2020.07554

Abstract Many attempts on optimization of sorghum [Sorghum bicolor (L.) Moench] tissue culture induction media have been made, but the culture system remains with some bottlenecks compared to that of other crops. This study aimed at assessing the suitability of various induction media to produce embryogenic callus (yellow and friable) with high induction rates and reduced phenolic exudation. The six culture medium modifications: 3 based on Murashige and Skoog (MS) medium and one each based on Chu N6, Gamborg B5 and 190-2 media respectively were applied in the culture of mature embryos from 10 sorghum genotypes. Although there was a genotype… More >