Na Liu1, Xiaoyun Zhang2, Yuzhen Ding2, Hong Li2, Xiumei Guan2, Min Cheng2,*, Xiaodong Cui2,*
Molecular & Cellular Biomechanics, Vol.16, Suppl.1, pp. 87-87, 2019, DOI:10.32604/mcb.2019.05751
Abstract Objective: Endothelial progenitor cells (EPCs) play a vital role in postnatal vascular injury and repair, especially vasculogenesis and angiogenesis. The purpose of this study was to investigate the effect of laminar shear stress in attenuating the decreased-expression of complement regulatory protein CD59 and the mechanism of cytoskeleton F-actin. Methods: EPCs were isolated from human umbilical vein blood and planted on glass slides, which applied to the laminar shear stress force (12 dyne/cm2) in a high glucose (20 mM) culture environment. The gene and protein expression of CD59 were detected by SYBGreen quantitative PCR and fluorescence activated cell sorter (FACS) respectively.… More >
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