Jifeng Li1,#, Yanting He1,#, Xiaoyun Zhang1, Hong Li1, Xiumei Guan1, Min Cheng1,*, Xiaodong Cui1,*
Molecular & Cellular Biomechanics, Vol.16, Suppl.1, pp. 89-89, 2019, DOI:10.32604/mcb.2019.05753
Abstract Objective: The propose of the study is to investigate the specific effects of the mechanically sensitive channel Kir2.1 on the transdifferentiation of EPCs so as to understand the molecular mechanism of pathological vascular remodeling. Methods: Endothelial progenitor cells (EPCs) were isolated from rat bone marrow and cultured in EGM2 medium in vitro. The recombinant lentiviral vectors carrying Kir2.1 (NM_017296.1) gene was designed and constructed in order to overexpress the gene. The smooth muscle cells (SMCs) molecules marker on EPCs, such as α-SMA, FSP1 and α-SM22, were detected by RT-PCR and cellular immunofluorescence. In addition, cell angiogenic capacity and migration in… More >
Login
Register
