HONG FU¹, HAOYU ZHAO¹, YALI YANG¹, SIYU WANG¹, KE DUAN^2,*, TAILIN GUO^1,*

BIOCELL, Vol.46, No.4, pp. 1067-1078, 2022, DOI:10.32604/biocell.2022.017435

Abstract CDK5 belongs to the cyclin-dependent kinase family. CDK5 is multifunctional and plays an important role in neural differentiation. However, the role of CDK5 in osteoblastic differentiation remains unclear. The present study investigated functions and molecular mechanism of CDK5 in osteoblastic differentiation. It was found that, CDK5 inhibition promoted the expression of Runx2, ALP, OCN and OPN of MSCs and the mineralization of MC-3T3E1 cells and MSCs. CDK5 inhibition enhanced the development of F-actin, nuclear localization of β-catenin and YAP, as well as the expression of RMRP RNA. When F-actin was suppressed by Blebbistatin, the nuclear localization of YAP and β-catenin,… More >

FRANCISCO CAPANI¹, EZEQUIEL SARACENO¹, VALERIA ROMINA BOTI¹, LAURA AON-BERTOLINO¹, JUAN CARLOS FERNÁNDEZ¹, FERNANDO GATO¹, MARIA SOL KRAUSE², LISANDRO GIRALDEZ³, MARK H. ELLISMAN⁴, HÉCTOR COIRINI^1,2

BIOCELL, Vol.32, No.1, pp. 1-8, 2008, DOI:10.32604/biocell.2008.32.001

Abstract Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a… More >

Danyang Yue¹, Yijuan Fan¹, Juan Lu¹, Mengxue Zhang¹, Jin Zhou¹, Yuying Bai¹, Jun Pan^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 144-144, 2019, DOI:10.32604/mcb.2019.07084

Abstract Mesenchymal Stem Cells (MSCs) are recruited to the musculoskeletal system following trauma [1] or chemicals stimulation [2]. The regulation of their differentiation into either bone or cartilage cells is a key question. The fluid shear stress (FSS) is of pivotal importance to the development, function and even the repair of all tissues in the musculoskeletal system [3]. We previously found that MSCs are sensitive enough to distinguish a slight change of FSS stimulation during their differentiation commitment to bone or cartilage cells, and the internal mechanisms. In detail, MSCs were exposed to laminar FSS linearly increased from 0 to 10… More >

Na Liu¹, Xiaoyun Zhang², Yuzhen Ding², Hong Li², Xiumei Guan², Min Cheng^2,*, Xiaodong Cui^2,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.1, pp. 87-87, 2019, DOI:10.32604/mcb.2019.05751

Abstract Objective: Endothelial progenitor cells (EPCs) play a vital role in postnatal vascular injury and repair, especially vasculogenesis and angiogenesis. The purpose of this study was to investigate the effect of laminar shear stress in attenuating the decreased-expression of complement regulatory protein CD59 and the mechanism of cytoskeleton F-actin. Methods: EPCs were isolated from human umbilical vein blood and planted on glass slides, which applied to the laminar shear stress force (12 dyne/cm2) in a high glucose (20 mM) culture environment. The gene and protein expression of CD59 were detected by SYBGreen quantitative PCR and fluorescence activated cell sorter (FACS) respectively.… More >

CHI, Maoru¹, ZHANG, Weihua¹ JIANG, Yiping¹ DAI, Huanyun¹

The International Conference on Computational & Experimental Engineering and Sciences, Vol.7, No.3, pp. 141-145, 2008, DOI:10.3970/icces.2008.007.141

Abstract This article has no abstract. More >

Wei Huang^∗,†, Yi Wah Mak^*, Peter C. Y. Chen^‡§

Molecular & Cellular Biomechanics, Vol.8, No.2, pp. 91-104, 2011, DOI:10.3970/mcb.2011.008.091

Abstract A molecular configuration tensor Pij was introduced to analyze the distribution of fibrous proteins in vascular cells for studying cells and tissues biomechanics. We have used this technique to study the biomechanics of vascular remodeling in response to the changes of blood pressure and flow. In this paper, the remodeling of the geometrical arrangement of F-actin fibers in the smooth muscle cells in rat's pulmonary arteries in hypoxic hypertension was studied. The rats were exposed to a hypoxia condition of 10% for 0, 2, 12, and 24 hr at sea level. Remodeling of blood vessels were studied at the in… More >

Wei Huang^*

Molecular & Cellular Biomechanics, Vol.4, No.3, pp. 119-132, 2007, DOI:10.3970/mcb.2007.004.119

Abstract This work presents a tensorial description of the geometrical arrangement of the cellular molecules in the vascular endothelial cells. The geometrical arrangement of the molecules is the foundation of the mechanical properties of the molecular aggregates, which are the foundation of the physical behavior of the cells and tissues. For better studying the physical behavior of the cells and tissues, the geometrical arrangement of the cellular molecules has to be described quantitatively. In this paper, a second order molecular configuration tensor P_ij^g for fibrous protein in the cells is defined for quantitative measurement. Here, the subscripts i, j refer… More >