FRANCISCO CAPANI1, EZEQUIEL SARACENO1, VALERIA ROMINA BOTI1, LAURA AON-BERTOLINO1, JUAN CARLOS FERNÁNDEZ1, FERNANDO GATO1, MARIA SOL KRAUSE2, LISANDRO GIRALDEZ3, MARK H. ELLISMAN4, HÉCTOR COIRINI1,2
BIOCELL, Vol.32, No.1, pp. 1-8, 2008, DOI:10.32604/biocell.2008.32.001
Abstract Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a… More >
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