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    In vitro evaluation of native entomopathogenic fungi and neem (Azadiractha indica) extracts on Spodoptera frugiperda

    Hernandez-Trejo A1, B Estrada-Drouaillet1, JA López-Santillán1, C Rios-Velasco2, SE Varela-Fuentes1, R Rodríguez-Herrera3, E Osorio-Hernández1

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 47-54, 2019, DOI:10.32604/phyton.2019.04611

    Abstract The control of Spodoptera frugiperda is based on synthetic insecticides, so some alternatives are the use of entomopathogenic fungi (EF) and neem extract. The objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on S. frugiperda larvae. Six EF were identified by DNA sequencing of ITS regions from three EF (Fusarium solani, Metarrhizium robertsii, Nigrospora spherica and Penicillium citrinum). They were evaluated in concentrations of 1 × 10⁸ spores/ mL. In addition, a second bioassay was carried out evaluating only F. solani, M. robertsii and N. sphaerica and the addition of vegetable oil.… More >

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