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  • Open Access

    ARTICLE

    Rapid Identification of a Candidate Gene Related to Fiber Strength Using a Superior Chromosome Segment Substitution Line from Gossypium hirsutum × Gossypium barbadense via Bulked Segregant RNA-Sequencing

    Qi Zhang1,#, Pengtao Li2,#, Aiying Liu1, Shaoqi Li1, Quanwei Lu2, Qun Ge1, Junwen Li1, Wankui Gong1, Xiaoying Deng1, Haihong Shang1,3, Yuzhen Shi1,*, Youlu Yuan1,3,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 837-858, 2021, DOI:10.32604/phyton.2021.014437

    Abstract Cotton is the most widely cultivated commercial crop producing natural fiber around the world. As a critical trait for fiber quality, fiber strength principally determined during the secondary wall thickening period. Based on the developed BC5F3:5 CSSLs (chromosome segment substitution lines) from Gossypium hirsutum CCRI36 × G. barbadense Hai 1, the superior MBI9915 was chosen to construct the secondary segregated population BC7F2 with its recurrent parent CCRI36, which was subsequently subjected to Bulk segregant RNA-sequencing (BSR-seq) for rapid identification of candidate genes related to fiber strength. A total of 4 fiber-transcriptome libraries were separately constructed and sequenced, including two parents… More >

  • Open Access

    ARTICLE

    A Multiscale Progressive Failure Modeling Methodology for Composites That Includes Fiber Strength Stochastics

    Trenton M. Ricks1, Thomas E. Lacy, Jr.1,2, Brett A. Bednarcyk3, Steven M.Arnold3, John W. Hutchins1

    CMC-Computers, Materials & Continua, Vol.40, No.2, pp. 99-130, 2014, DOI:10.3970/cmc.2014.040.099

    Abstract A multiscale modeling methodology was developed for continuous fiber composites that incorporates a statistical distribution of fiber strengths into coupled multiscale micromechanics/ finite element (FE) analyses. A modified twoparameter Weibull cumulative distribution function, which accounts for the effect of fiber length on the probability of failure, was used to characterize the statistical distribution of fiber strengths. A parametric study using the NASA Micromechanics Analysis Code with the Generalized Method of Cells (MAC/GMC) was performed to assess the effect of variable fiber strengths on local composite failure within a repeating unit cell (RUC) and subsequent global failure. The NASA code FEAMAC… More >

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